We narrowed to 18,247 results for: multi
-
Plasmid#103178PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-10b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-10b-3p target (MIR10B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-10b-5p
Plasmid#103179PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-10b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-10b-5p target (MIR10B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7g-3p
Plasmid#103157PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7g-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7g-3p target (MIRLET7G Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7g-5p
Plasmid#103158PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7g-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7g-5p target (MIRLET7G Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1-5p
Plasmid#103162PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-100-3p
Plasmid#103163PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-100-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-100-3p target (MIR100 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
ExpressionBacterial and YeastAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-dAPEX2
Plasmid#117174PurposePeroxidase reporter for multiplexed electron microscopy labelingDepositorInsertdAPEX2
UseAAV and Cre/LoxExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330A-1x3
Plasmid#58767PurposeExpresses Cas9 nuclease and gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163058PurposeroGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorInsertroGFP2-Orp1
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ173
Plasmid#99907PurposeExpress CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertpco-dCas9-VP64-T2A-MS2-VP64
UseCRISPRExpressionPlantAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU41
Plasmid#167663PurposeENTRY vector for gRNA1 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSN501
Plasmid#50589PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSMutationCas9D10A nickase, was derived from the zCas9 and …Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Renilla
Plasmid#118066PurposeTranscriptional unit for constitutive expression of Renilla in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV:GreenRenilla:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-DIO-COX4-dAPEX2
Plasmid#117177PurposePlasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAV and Cre/LoxExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMy-CTCF-GFP
Plasmid#50574PurposeRetroviral vector endoding for mouse CTCF fused to GFPDepositorInsertAmpicilin
UseRetroviralAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only