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pHR V5-RNF25_IRES-mCherry
Plasmid#198386PurposeRNF25 expressionDepositorInsertRNF25 (RNF25 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP680-P2A-GFP
Plasmid#197164PurposeExpresses the protein of miRFP680-P2A-GFP in mammalian cellsDepositorInsertmiRFP680-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABEmax
Plasmid#119815PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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hIRE1a K599A
Plasmid#20745DepositorInsertIRE1 (ERN1 Human)
UseLentiviral; Destination vectorTagsExpressionMammalianMutationK599A, dominant negative kinase mutantPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-H2B-mEGFP
Plasmid#105528PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNADepositorInsertH2B (H2BC11 Human)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-emiRFP670-P2A-GFP
Plasmid#197160PurposeExpresses the protein of emiRFP670-P2A-GFP in mammalian cellsDepositorInsertemiRFP670-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p4489 Flag-betaTrCP
Plasmid#10865DepositorInsertbetaTrCP (BTRC Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Blast
Plasmid#227260PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-AAVS1
Plasmid#227272PurposeExpresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.DepositorInsertsgRNA Targeting AAVS1 (AAVS1 Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-FLAG-CD59-GPI
Plasmid#50378PurposeExpress mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cellsDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-TJP1
Plasmid#227299PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of TJP1 (TJP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
aav-tnt-gfp-v2
Plasmid#165036PurposeAAV-based delivery of GFP into cardiomyocytes in vivoDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Circular guide cloning vector
Plasmid#180184PurposeCloning vector for expressing circular RNA from a U6 promoterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PPARgamma2
Plasmid#78768PurposeTo overexpress PPARgamma2 in Mammalian CellsDepositorInsertPPARG (Pparg Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-2A-3xFLAG-XBP1u_pause-2A
Plasmid#159583PurposeLuciferase reporter for ribosome pausingDepositorInsertsXBP1u pause site
EIF2S3 5UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano65/pcDNA3
Plasmid#51965Purposemammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nMDepositorInsertYellow Cameleon-Nano65
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-2A-3xFLAG-SBP-2A
Plasmid#159584PurposeLuciferase reporter for ribosome pausing controlDepositorInserts2A-3xFLAG-SBP-2A
EIF2S3 5UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only
