165,214 results

1. FLAG-HSF1

   Plasmid

   #32537

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV8R-12 Capsid

   Plasmid

   #213968

   Purpose

   AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO/cmycDrosha

   Plasmid

   #10828

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

   Insert

   Drosha (DROSHA Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. scAAV-hIBA1a-GFP

   Plasmid

   #214146

   Purpose

   Self-complementary AAV vector packaging GFP under the human Iba1 promoter

   Depositor

   Chun-Li Zhang

   Article

   Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

   Insert

   GFP
   

   Use

   AAV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Promoter

   U6, Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_GPHN.FingR-eGFP-CCR5TC

   Plasmid

   #46296

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.TBG.PI.eGFP.WPRE.bGH

   Plasmid

   #105535

   Purpose

   AAV expression of EGFP from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TBG

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-TOM20_Halo_T2A_EGFP

   Plasmid

   #135443

   Purpose

   Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   TOM20-HaloTag7 and EGFP
   

   Tags

   TOM20

   Expression

   Mammalian

   Promoter

   CMV/TetO

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP Centrin2 (Nigg UK185)

   Plasmid

   #41147

   Depositor

   Erich Nigg

   Article

   Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

   Insert

   Centrin-2 (CETN2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUtdTW

    Plasmid

    #22478

    Depositor

    Connie Cepko

    Article

    Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    tandem dimer tomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-GFP-APEX2-NES_pLX208

    Plasmid

    #202018

    Purpose

    expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-GFP-APEX2-NES
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNeonGreen-mTurquoise2

    Plasmid

    #98886

    Purpose

    Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-SFB

    Plasmid

    #99391

    Purpose

    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

    Plasmid

    #198337

    Purpose

    PB plasmid expressing Halo-tagged PP7 coat protein

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    PP7 coat protein 3x Halo-GB1
    

    Use

    Piggybac

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

    Plasmid

    #190162

    Purpose

    Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

    Insert

    mcherry_(3'UTR, No Actin)_24Broccoli
    

    Tags

    24×Broccoli

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid

    #108542

    Purpose

    Lentivirus vector expressing Luciferase with blasticidin resistance

    Depositor

    Javier Alcudia

    Inserts

    Luciferase
    Neomycin
    

    Use

    Lentiviral

    Promoter

    EF1apha and PGK

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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