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pBEL1198

Plasmid

#162134

Purpose

Expression and complementation plasmid for MlaFEDCB, with N-terminal his tag on MlaE

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2093

Plasmid

#162135

Purpose

Expression and Complementation plasmid for MlaE IF1 1-15 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-15)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2132

Plasmid

#162136

Purpose

Expression and Complementation plasmid for MlaE IF1 1-25 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-25)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2092

Plasmid

#162137

Purpose

Expression and Complementation plasmid for MlaE IF1 1-30 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-30)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2133

Plasmid

#162138

Purpose

Expression and Complementation plasmid for MlaE IF1 1-39 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-39)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2099

Plasmid

#162139

Purpose

Complementation plasmid for MlaE Y81A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(Y81A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2100

Plasmid

#162140

Purpose

Complementation plasmid for MlaE Y81W

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(Y81W)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2098

Plasmid

#162141

Purpose

Complementation plasmid for MlaE R97A

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(R97A)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2414

Plasmid

#175966

Purpose

Genetic complementation plasmid of LetAB with LetB(P701N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

P701N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2415

Plasmid

#175967

Purpose

Genetic complementation plasmid of LetAB with LetB(I703N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

I703N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2416

Plasmid

#175968

Purpose

Genetic complementation plasmid of LetAB with LetB(A705N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

A705N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2417

Plasmid

#175969

Purpose

Genetic complementation plasmid of LetAB with LetB(A706N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

A706N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2418

Plasmid

#175970

Purpose

Genetic complementation plasmid of LetAB with LetB(G707N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

G707N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2419

Plasmid

#175971

Purpose

Genetic complementation plasmid of LetAB with LetB(V708N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

V708N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2420

Plasmid

#175972

Purpose

Genetic complementation plasmid of LetAB with LetB(P717N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

P717N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1683

Plasmid

#181816

Purpose

Expression plasmid for LetB ΔRing 2 (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetB (a.k.a. b1834, yebT, ECK1833)

Expression

Bacterial

Mutation

LetB(43-877; delta160-279)-6xHis

Promoter

T7

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2307

Plasmid

#181817

Purpose

Expression plasmid for LetB ΔRing 3 (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetB (a.k.a. b1834, yebT, ECK1833)

Expression

Bacterial

Mutation

LetB(43-877; delta280-391)-6xHis

Promoter

T7

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2308

Plasmid

#181818

Purpose

Expression plasmid for LetB ΔRing 4 (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetB (a.k.a. b1834, yebT, ECK1833)

Expression

Bacterial

Mutation

LetB(43-877; delta392-515)-6xHis

Promoter

T7

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2309

Plasmid

#181819

Purpose

Expression plasmid for LetB ΔRing 5 (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetB (a.k.a. b1834, yebT, ECK1833)

Expression

Bacterial

Mutation

LetB(43-877; delta516-633)-6xHis

Promoter

T7

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1749

Plasmid

#181820

Purpose

Expression plasmid for LetB ΔRing 7 (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetB (a.k.a. b1834, yebT, ECK1833)

Expression

Bacterial

Mutation

LetB(43-877; delta747-877)-6xHis

Promoter

T7

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1748

Plasmid

#181815

Purpose

Expression plasmid for LetB ΔRing 1 (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetB (a.k.a. b1834, yebT, ECK1833)

Expression

Bacterial

Mutation

LetB(43-877; delta48-159)-6xHis

Promoter

T7

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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prrnBT1_STD

Plasmid

#165803

Purpose

Bacterial rrnB gene T1 terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

rrnBT1

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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prrnBT2_STD

Plasmid

#165804

Purpose

Bacterial rrnB gene T2 terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

rrnBT2

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHPH

Plasmid

#165839

Purpose

hygromycin B resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

hph

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBLE

Plasmid

#165840

Purpose

Zeocin/Phleomycin resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

ble

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNPTII

Plasmid

#165836

Purpose

G418 sulfate (geneticin) resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

nptII

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAC

Plasmid

#165837

Purpose

puromycin resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

pac

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pspy_STD

Plasmid

#165806

Purpose

bacterial terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

spy

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2039

Plasmid

#139929

Purpose

Complementation plasmid for LetAB without LetB TM region

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(43-877)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-wtBlc

Plasmid

#168255

Purpose

wild‐type E. coli lipocalin Blc without signal peptide

Depositor

Jens Meiler

Article

Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

Insert

Lipocalin Blc

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge