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pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

Plasmid

#114091

Purpose

CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized AsCas12a (E174R/S542R)

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

E174R and S542R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

Plasmid

#114083

Purpose

CAG promoter expression plasmid for human codon optimized enAsBE1.3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)

Tags

SV40 NLS

Expression

Mammalian

Mutation

E174R, S542R, K548R and D908A, human codon optimi…

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

Plasmid

#114080

Purpose

CAG promoter expression plasmid for human codon optimized AsBE1.4

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)

Tags

2x SV40 NLS and SV40 NLS

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pXJ512-pAAV-PB3'IR-CAG-MCP-mtagBFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254952

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ514-pAAV-PB3'IR-CAG-PCP-mScarlet-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254953

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ515-pAAV-PB3'IR-CAG-PCP-mtagBFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254954

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ520-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-hU6-gRNA-PB5'IR

Plasmid

#254955

Purpose

AAV piggyBac vector with single gRNA and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ521-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-hU6-gRNA-PB5'IR

Plasmid

#254956

Purpose

AAV piggyBac vector with single gRNA and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ494-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254949

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-GFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ496-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254950

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ511-pAAV-PB3'IR-CAG-MCP-mScarlet-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254951

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-GCaMP6s

Plasmid

#241023

Purpose

An HDR plasmid for the AAVS1 safe harbor locus carrying a gene cassette consisting of GCaMP6s under control of a CAG promoter and a WPRE.

Depositor

Thomas Johnson

Article

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s and WPRE

Use

CRISPR

Tags

6x His Tag

Promoter

CAG

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-eYFP(nuclear)-2A-N2cG

Plasmid

#248664

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear eYFP and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-eYFP

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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FRB-mUSH1C-DFCR-pEGFP

Plasmid

#251431

Purpose

Mammalian expression vector for expressing the actin-binding motif of mouse harmonin b with an N-terminal FRB and a C-terminal EGFP

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

The DFCR fragment (residues 296-728 of Ush1C) (Ush1c Mouse)

Tags

EGFP and FRB

Expression

Mammalian

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Galntl5 (37 kDa)-3xHA

Plasmid

#240646

Purpose

The nucleotides coding 37 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Galntl5 (30 kDa)-3xHA

Plasmid

#240647

Purpose

The nucleotides coding 30 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Galntl5 (20 kDa)-3xHA

Plasmid

#240648

Purpose

The nucleotides coding 20 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Galntl5 (10 kDa)-3xHA

Plasmid

#240649

Purpose

The nucleotides coding 10 kDa from the C-terminus of mouse Galntl5 variant #1 and 3xHA tag sequence were inserted under the CAG promoter.

Depositor

Taichi Noda

Article

Noda et al Nat Commun. 2025 Sep 17;16(1):8264. doi:

Insert

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 5 (Galntl5 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GRN-HA

Plasmid

#243760

Purpose

AAV plasmid expressing human GRN with a C-terminal HA tag under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Progranulin (GRN Human)

Use

AAV

Tags

HA Tag

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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