We narrowed to 5,474 results for: chia

1. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132407

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLwaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R474A; H479A; R1046A; R1051A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWPSG-Ptet-nCas9-BE

   Plasmid

   #195739

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet(Tetracycline inducible protein)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD001 pME minprom_EGFP

   Plasmid

   #195955

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann

   Article

   Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

   Insert

   mouse beta-globin minimal promoter EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF-p15A-YFP

   Plasmid

   #186563

   Purpose

   p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   None

   Expression

   Bacterial and Mammalian

   Mutation

   None

   Promoter

   Promotorless

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b CnA CnB

   Plasmid

   #11787

   Depositor

   Anjana Rao

   Article

   Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

   Insert

   CnA CnB (PPP3R1, PPP3CA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. p23-NES-LwaCas13a-msfGFP-NES-Flag

   Plasmid

   #165070

   Purpose

   overexpression of LwaCas13a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   LwaCas13a
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWPSG-Po-nCas9-BE

   Plasmid

   #195740

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Po (phoenol-inducible promoter)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4-ERSE2-luc2P-Hygro

    Plasmid

    #101790

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-II
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. NgOE FUNgGW

    Plasmid

    #92231

    Purpose

    Lentiviral expression of human Ng

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    Ng (NRGN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBa.GFP-KIF5B

    Plasmid

    #134625

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99A-Rat FABP1

    Plasmid

    #13577

    Depositor

    Martin Scanlon

    Article

    Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

    Insert

    Rat liver fatty acid binding protein (Fabp1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Use

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJBEI-6411

    Plasmid

    #47050

    Purpose

    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. CamKIIa:smFP-TVA-RGV

    Plasmid

    #202594

    Purpose

    Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

    Depositor

    Oleksandr Shcheglovitov

    Article

    Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

    Inserts

    smFP
    TVA
    RGV
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAPDH-Cre-HYG

    Plasmid

    #231510

    Purpose

    Expresses Cre recombinase in Acanthamoeba

    Depositor

    Yeonchul Hong

    Insert

    Cre recombinase
    

    Use

    Unspecified; Acanthamoeba castellanii neff strain

    Tags

    EGFP and SV40 NLS

    Expression

    Mutation

    Promoter

    Acanthamoeba GAPDH

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. NgΔIQ FUNgDIQGW

    Plasmid

    #92232

    Purpose

    Lentiviral expression of human Ng with deletion mutation

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    Ng (NRGN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta IQ

    Promoter

    hUbC

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORTMAGE-1

    Plasmid

    #72680

    Purpose

    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    lower strength RBS before mutL E32K allele than i…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only