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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

Plasmid

#55646

Purpose

Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p5E-gfap

Plasmid

#82401

Purpose

Gateway-compatible zebrafish gfap promoter

Depositor

Judith Eisen

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

gfap promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFA6a-LEU2MX6

Plasmid

#33138

Purpose

gene deletion in S. cerevisiae and S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Insert

LEU2MX6

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ141A

Plasmid

#69290

Purpose

Gateway entry vector; single gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Publication

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Expression

Bacterial

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLXSN18E6E7

Plasmid

#53459

Purpose

The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.

Depositor

Denise Galloway

Publication

Halbert et al J Virol. 1991 Jan;65(1):473-8. doi: 10.1

Insert

HPV18E6E7

Use

Retroviral

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Publication

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Expression

Bacterial and Yeast

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

Plasmid

#68714

Depositor

Yutaka Hata

Insert

H2B mCherry and GFP

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Publication

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pYLsgRNA-OsU6a

Plasmid

#66194

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6a

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRU43

Plasmid

#167665

Purpose

ENTRY vector for gRNA3 cloning under pAtU6 promoter

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Publication

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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SID4x-dCas9-KRAB

Plasmid

#106399

Purpose

Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

Depositor

Jason Gertz

Publication

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Inserts

SID4x (MXD1 Human)
KRAB (ZNF10 Human)

Tags

FLAG, HA, and NLS

Expression

Mammalian

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSPORT-RREB1

Plasmid

#41145

Depositor

Joshua Mendell

Publication

Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

Insert

RREB-1 (RREB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIPZ (M)-HT-Cbx2

Plasmid

#82510

Purpose

Expresses Halotag-Cbx2 fusion proteins in mammalian cells

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 2 (CBX2 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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