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p15aC-3D1D-5

Plasmid

#121050

Purpose

Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K

Tags

myc and none

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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LC402: pMVP (L3-L2) eGFP + polyA

Plasmid

#121763

Purpose

pMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KG802: pMVP (L3-L2) V5 epitope tag + polyA

Plasmid

#121755

Purpose

pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

V5 epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0119

Plasmid

#177051

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

major latex protein-like (NmMLPL) from Nepeta mussinii

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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KW701: pMVP (L5-L4) AID

Plasmid

#121723

Purpose

pMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

AID (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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II901: pMVP (L5-L4) FKBP DD

Plasmid

#121720

Purpose

pMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

FKBP Degron Domain (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3xFlag-S242R-MEFV

Plasmid

#167020

Purpose

Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Mutation

serine 242 in arginine (S242R)

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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JN402: pMVP (R4-R3) Firefly Luciferase

Plasmid

#121741

Purpose

pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Firefly Luciferase

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

Plasmid

#162918

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase/green fluorescent protein fusion with upstream IRES sequence

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S103L (#624)

Plasmid

#99942

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S103L

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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II801: pMVP (L5-L4) ecDHFR DD

Plasmid

#121721

Purpose

pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

ecDHFR Degron Domain (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H02

Plasmid

#70956

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Iswi (Iswi Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SFFV L1-R5

Plasmid

#62097

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV/TO L1-R5

Plasmid

#62099

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A-SRDX

Plasmid

#129684

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b-SRDX

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV/TO R4-R3

Plasmid

#62100

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6-miR-30 L5-L2

Plasmid

#62115

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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