We narrowed to 12,677 results for: sequence

1. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

   Plasmid

   #149347

   Purpose

   Mammalian expression vector for SpyCas9 and puromycin resistance

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

   Plasmid

   #149350

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMJB1-RPL1B-Cl

   Plasmid

   #160430

   Purpose

   Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   RPL1B
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Removed introns, shuffled codons to vary nucleoti…

   Promoter

   S. cerevisiae GAL1

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Linear-1_PolyU(1)

   Plasmid

   #159479

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-U6+27-Linear-1_PolyU(2)

   Plasmid

   #159480

   Purpose

   Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV-U6+27-Linear-1_PolyU(3)

   Plasmid

   #159481

   Purpose

   Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ265H

   Plasmid

   #149554

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   hAID-ziSpyMacCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ266B

   Plasmid

   #149555

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   ziSpyMacCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMX-eSOX2AHK

   Plasmid

   #118826

   Purpose

   Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

   Depositor

   Ralf Jauch

   Article

   Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

   Insert

   eSox2AHK (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   AHK: E46A, I53H

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMX-eSOX2NRR

    Plasmid

    #118825

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX2NRR (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NRR: E46N, I53R, K57R

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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