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We narrowed to 12,157 results for: pcDNA

Showing: 7251 - 7300 of 12157 results
  1. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Expression
    Mammalian
    Promoter
    CMV and NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

    Plasmid
    #155010
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)

    Plasmid
    #52133
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and C478S, C631S, C643S,…
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid
    #50914
    Purpose
    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C I730C N-term deletion of aa13-222 in hNKCC1…
    Promoter
    CMV
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid
    #50865
    Purpose
    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C C723S C724V A735C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid
    #50913
    Purpose
    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S150N S155Q S170Q T177A S183R S193E T203A T205A T…
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid
    #198914
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid
    #198451
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid
    #182686
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.
    Insert
    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3

    Plasmid
    #198915
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector
    Depositor
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    snp rs2278973 Arginine
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3

    Plasmid
    #198916
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector
    Depositor
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174646
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    CMV (with TetOn)
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid
    #205703
    Purpose
    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines
    Depositor
    Insert
    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
  18. raft-APEX2

    Plasmid
    #205089
    Purpose
    Expresses lipid-raft targeted APEX2 for proximity proteomics. Vector: pcDNA™4/TO. Insert: lipid raft target sequence (MGCVCSS) + mCherry +V5 (GKPIPNPLLGLDST) +APEX2
    Depositor
    Insert
    APEX2 (raft-mCherry-V5 tagged)
    Tags
    lipid raft targeting sequence (MGCVCSS), mCherry,…
    Expression
    Mammalian
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pOME0296M

    Plasmid
    #182625
    Purpose
    Reverse sequence of KSHV Kaposincluster-RevCompl-Syn for pcDNA
    Depositor
    Insert
    KSHV Kaposincluster_RevCompl_SYN_rev
    Expression
    Mammalian
    Available Since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. pPD765 TRE3GV MCS in TU1

    Plasmid
    #139253
    Purpose
    TRE3GV promoter and an MCS in TUPV1
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pPD767 TRE3GV MCS in TU4

    Plasmid
    #139256
    Purpose
    TRE3GV promoter and an MCS in TUPV4
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. g15s pD47

    Plasmid
    #117464
    Purpose
    Expression of PSD2
    Depositor
    Insert
    PSD2 (PSD2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  23. pPD1159 pLink 9

    Plasmid
    #139247
    Purpose
    Vector that holds the linker for circuits with 9 TUs
    Depositor
    Insert
    Linker9
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pPD1158 pLink 8

    Plasmid
    #139246
    Purpose
    Vector that holds the linker for circuits with 8 TUs
    Depositor
    Insert
    Linker8
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. pPD1157 pLink 7

    Plasmid
    #139245
    Purpose
    Vector that holds the linker for circuits with 7 TUs
    Depositor
    Insert
    Linker7
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  26. pPD1156 pLink 6

    Plasmid
    #139244
    Purpose
    Vector that holds the linker for circuits with 6 TUs
    Depositor
    Insert
    Linker6
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  27. pPD1155 pLink 5

    Plasmid
    #139243
    Purpose
    Vector that holds the linker for circuits with 5 TUs
    Depositor
    Insert
    Linker5
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  28. pPD1154 pLink 4

    Plasmid
    #139242
    Purpose
    Vector that holds the linker for circuits with 4 TUs
    Depositor
    Insert
    Linker4
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  29. pPD1153 pLink 3

    Plasmid
    #139241
    Purpose
    Vector that holds the linker for circuits with 3 TUs
    Depositor
    Insert
    Linker3
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  30. pPD1152 pLink 2

    Plasmid
    #139240
    Purpose
    Vector that holds the linker for circuits with 2 TUs
    Depositor
    Insert
    Linker2
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  31. pPD1151 pLink 1

    Plasmid
    #139239
    Purpose
    Vector that holds the linker for circuits with 1 TUs
    Depositor
    Insert
    Linker1
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. g15s pD42

    Plasmid
    #117465
    Purpose
    Expression of PSD2
    Depositor
    Insert
    PSD2 (PSD2 Human)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  33. g11s pD42

    Plasmid
    #117469
    Purpose
    Expression of PSD3
    Depositor
    Insert
    PSD3 (PSD3 Human)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  34. pCMV-mPlum-IFP fusion

    Plasmid
    #49768
    Purpose
    For near infrared in vivo optical imaging
    Depositor
    Insert
    mPlum-IFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
  35. pHIE096 CMV Bxb1

    Plasmid
    #139252
    Purpose
    Encodes a Bxb1 recombinase for Landing Pad integration.
    Depositor
    Insert
    Bxb1 Recombinase
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  36. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid
    #139254
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV2
    Depositor
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. pJM467 TRE3GV EYFP in TU3

    Plasmid
    #139255
    Purpose
    TRE3GV promoter and EYFP in TUPV3
    Depositor
    Insert
    EYFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  38. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid
    #139257
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV5
    Depositor
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  39. pCMV-AAVS1-TALEN-L

    Plasmid
    #159299
    Purpose
    TALEN (left) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  40. pCMV-AAVS1-TALEN-R

    Plasmid
    #159300
    Purpose
    TALEN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Insert
    TALEN targeting the AAVS1
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  41. pPD698 ZF172i

    Plasmid
    #138791
    Purpose
    Constitutive expression of ZF172i under the CMV promoter.
    Depositor
    Insert
    ZF172i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  42. pPD699 ZF173i

    Plasmid
    #138792
    Purpose
    Constitutive expression of ZF173i under the CMV promoter.
    Depositor
    Insert
    ZF173i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  43. pPD686 ZF13i

    Plasmid
    #138774
    Purpose
    Constitutive expression of ZF13i under the CMV promoter.
    Depositor
    Insert
    ZF13i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  44. pPD687 ZF14i

    Plasmid
    #138775
    Purpose
    Constitutive expression of ZF14i under the CMV promoter.
    Depositor
    Insert
    ZF14i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  45. pPD688 ZF21i

    Plasmid
    #138776
    Purpose
    Constitutive expression of ZF21i under the CMV promoter.
    Depositor
    Insert
    ZF21i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  46. pPD689 ZF36i

    Plasmid
    #138777
    Purpose
    Constitutive expression of ZF36i under the CMV promoter.
    Depositor
    Insert
    ZF36i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  47. pPD690 ZF42i

    Plasmid
    #138779
    Purpose
    Constitutive expression of ZF42i under the CMV promoter.
    Depositor
    Insert
    ZF42i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  48. pPD691 ZF54i

    Plasmid
    #138781
    Purpose
    Constitutive expression of ZF54i under the CMV promoter.
    Depositor
    Insert
    ZF54i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7251 - 7300 of 12157 results