We narrowed to 12,311 results for: BASE

1. pcDNA5/FRT/TO_eGFP-pScaE

   Plasmid

   #228826

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaE
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 49-494

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_eGFP-pScaD

   Plasmid

   #228825

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaD
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 30-700

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_eGFP-pScaC

   Plasmid

   #228824

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO_eGFP-pScaA

   Plasmid

   #228823

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaA
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 25-1216

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCN34e-yfp

   Plasmid

   #227641

   Purpose

   Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM198

   Plasmid

   #227657

   Purpose

   pRS305 His-HRV3C-Rpn5 WT

   Depositor

   Andreas Martin

   Article

   Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

   Insert

   rpn5 (RPN5 Budding Yeast)
   

   Use

   Tags

   His-HRV3C-

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM200

   Plasmid

   #227659

   Purpose

   pRS305 3XFLAG-HRV3C-Rpn5 WT

   Depositor

   Andreas Martin

   Article

   Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

   Insert

   rpn5 (RPN5 Budding Yeast)
   

   Use

   Tags

   3X FLAG-HRV3C-

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM202

   Plasmid

   #227661

   Purpose

   pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

   Depositor

   Andreas Martin

   Article

   Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

   Insert

   rpn5 (RPN5 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS2699-CMV-Nme2Cas9-E932R

   Plasmid

   #228720

   Purpose

   Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-E932R nuclease
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid

    #228717

    Purpose

    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-D56R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid

    #228718

    Purpose

    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-E932R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEJS2698-CMV-Nme2Cas9-D56R

    Plasmid

    #228719

    Purpose

    Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-D56R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB-CRISPRi

    Plasmid

    #221136

    Purpose

    Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    cbu1169 promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GST-beta catenin tag

    Plasmid

    #186422

    Purpose

    Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    GST-beta catenin tag
    

    Use

    Tags

    6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

    Plasmid

    #225442

    Purpose

    BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

    Depositor

    Diego Orzaez

    Insert

    LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE5417_p3WJdB_promoter dropout vector

    Plasmid

    #221191

    Purpose

    Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Promoter Placeholder/Dropout Vector

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-mTagBFP2

    Plasmid

    #220747

    Purpose

    mTagBFP2 expression in mammalian cells. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWR95(URA3)

    Plasmid

    #203917

    Purpose

    BLINCAR plasmid for gene deletion, URA3 target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    URA3 upstream homology
    URA3 downstream homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSuperCRISPR-sgRNA-RL661-TTTA

    Plasmid

    #215865

    Purpose

    This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

    Depositor

    Kumiko Ui-Tei

    Article

    Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

    Insert

    sgRNA-RL661 with TTTA stretch
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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