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pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

Plasmid

#60660

Purpose

Flp-dependent expression of mNaChBacMut-T2A-tdTomato

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX-mNaChBacMut-T2A-tdTomato

Use

AAV

Mutation

E191K

Promoter

EF1α

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0mut

Plasmid

#153290

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytes

Depositor

Yulong Li

Publication

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

GfaABC1D

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

Plasmid

#75082

Purpose

An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Publication

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Nuc-eYFP

Use

AAV and Cre/Lox

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Publication

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6

Plasmid

#170857

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_uTCR-KI

Plasmid

#257993

Purpose

AAV transfer plasmid cloning backbone for TCR knock-in mouse generation.

Depositor

Gabriel Victora

Publication

Bilanovic et al EMBO J. 2026 May 13. doi: 10.1038/s44318

Insert

Trbv16 promoter flanked by 5' and 3' homology arms to the endogenous Trbj2 locus

Available since

Aug. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-ChroME2s-GCaMP8s-ST

Plasmid

#258864

Purpose

AAV expression of some-targeted ChroME2f fused to GCaMP8s

Depositor

Hillel Adesnik

Insert

ChroME2s

Use

AAV

Tags

GCaMP8s

Expression

Mammalian

Promoter

TRE

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Cyp26a1

Plasmid

#185563

Purpose

AAV construct to ubiquitously express mouse Cyp26a1

Depositor

Connie Cepko

Publication

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

Cyp26a1 (Cyp26a1 Mouse)

Use

AAV

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG eGFP T2A SP HALO HAPLN1

Plasmid

#228200

Purpose

AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

Depositor

Sungjin Park

Publication

Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

Insert

HAPLN1 (Hapln1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

constitutive CAG promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-fCAMK-GFP

Plasmid

#22917

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-LACTB-dAPEX2

Plasmid

#117180

Purpose

Plasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Publication

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-EGFP-WPRE-ID-PolyA

Plasmid

#241829

Purpose

AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

Depositor

Benjamin Arenkiel

Publication

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

EGFP-WPRE-ID-PolyA

Use

AAV

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA18-GCaMP6s

Plasmid

#126004

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Promoter

ProA18

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCherry-LATeNT

Plasmid

#240989

Purpose

Expresses mCherry-LATeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-LATeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

Plasmid

#122102

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

Plasmid

#111391

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Publication

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA-mRuby2

Use

AAV

Tags

Myc and mRuby2

Expression

Mammalian

Promoter

hSynapsin

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pC178: pAAV.U6.SapI.CMV.Cas13bt3

Plasmid

#204215

Purpose

Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

Depositor

Guei-Sheung Liu

Publication

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

Cas13bt3 and U6.SapI sgRNA cloning site

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Codon optimisation by GenScript tool

Promoter

CMV/U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AiP15139 - pAAV-AiE0873m_3xC2-minBG-iCre(Q311R)-BGHpA

Plasmid

#243638

Purpose

AiE0873m_3xC2 is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

iCre(Q311R)

Use

AAV

Mutation

Mutation at 311th amino acid of iCre

Available since

Oct. 29, 2026

Availability

Academic Institutions and Nonprofits only

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