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We narrowed to 6,530 results for: nls

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Showing: 5841 - 5880 of 6530 results

  2. pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)

    Plasmid
    #242652
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, SpG mutations in SpCas9…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242652-plasmid-map-sequence-id-488985

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  3. pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971)

    Plasmid
    #242653
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpCas9-VRQR-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, VRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242653-plasmid-map-sequence-id-488991

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  4. pCMV-ABE8.8m-eVRQR-P2A-EGFP (BKS974)

    Plasmid
    #242655
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-VRQR(S55R)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, eVRQR mutations in SpCa…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242655-plasmid-map-sequence-id-488977

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  5. pCMV-ABEmax-eVRQR-P2A-EGFP (LLH575)

    Plasmid
    #242654
    Purpose
    CMV promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABEmax-VRQR-S55R-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE7.10 mutations in TadA, eVRQR mutations in SpC…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242654-plasmid-map-sequence-id-488975

    Enlarge

  7. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  8. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  9. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  11. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  12. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  14. Bxb1-GT-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

    Plasmid
    #241396
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103
    Insert
    TRE-Ngn2-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3, NEUROG2 Human)
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    TRE; CAG
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    241396-plasmid-map-sequence-id-489344

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  16. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  18. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  19. pPD8_33

    Plasmid
    #1468
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1468-plasmid-map-sequence-id-79

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  20. pPD16_43

    Plasmid
    #1433
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1433-plasmid-map-sequence-id-44

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  21. pPD26_77

    Plasmid
    #1441
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1441-plasmid-map-sequence-id-52

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  22. pPD90_23

    Plasmid
    #1469
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1469-plasmid-map-sequence-id-80

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  23. pDD16_01

    Plasmid
    #1428
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1428-plasmid-map-sequence-id-39

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  24. pPD16_51

    Plasmid
    #1434
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1434-plasmid-map-sequence-id-45

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  25. pPD18_32

    Plasmid
    #1435
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1435-plasmid-map-sequence-id-46

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  26. pPD21_28

    Plasmid
    #1438
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1438-plasmid-map-sequence-id-49

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  27. pPD8_02

    Plasmid
    #1467
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1467-plasmid-map-sequence-id-78

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  28. pPD22_04

    Plasmid
    #1439
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2204, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1439-plasmid-map-sequence-id-50

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  29. pPD22_11

    Plasmid
    #1440
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2211, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1440-plasmid-map-sequence-id-51

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  30. pPD81_34

    Plasmid
    #1457
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    rf8134, IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1457-plasmid-map-sequence-id-68

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  31. pPD18_49

    Plasmid
    #1436
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, myo3N, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1436-plasmid-map-sequence-id-47

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  32. pBK125

    Plasmid
    #1688
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-25', hsp16-415', SV40NLS, gfpN, g…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1688-plasmid-map-sequence-id-298

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  33. pPD91_14

    Plasmid
    #1472
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, gfp…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1472-plasmid-map-sequence-id-83

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  34. pPD50_14

    Plasmid
    #1449
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-415', hsp16-25', IVSA, SV40NLS, l…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1449-plasmid-map-sequence-id-60

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  35. L2963

    Plasmid
    #1528
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, IVS
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1528-plasmid-map-sequence-id-138

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  36. pPD20_97

    Plasmid
    #1437
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo2-1090, myo25', IVSA, SV40NLS, lacZN, lac…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1437-plasmid-map-sequence-id-48

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  37. pPD50_21

    Plasmid
    #1450
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    hsp16-25', hsp16-415', IVSA, SV40NLS, l…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1450-plasmid-map-sequence-id-61

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  38. pPD87_18

    Plasmid
    #1458
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, lacZN, la…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1458-plasmid-map-sequence-id-69

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  39. pPD88_22

    Plasmid
    #1460
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, lacZN, la…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1460-plasmid-map-sequence-id-71

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  40. pPD90_28

    Plasmid
    #1470
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, IVSE, IVSH, IVSK, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1470-plasmid-map-sequence-id-81

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Showing: 5841 - 5880 of 6530 results