We narrowed to 9,185 results for: Pol;

1. pBM23-pyrF-CS

   Plasmid

   #174396

   Purpose

   pBM23 contained pyrF and mini-CRISPR.

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   pyrF-CS
   

   Use

   Template for cloning fragments for chromosomal in…

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CTAG_linker-eGFP

   Plasmid

   #136098

   Purpose

   FP for C-term fusion with CDS12 without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG_AlaSerGly10xAlaGlySer_eGFP
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CTAG_linker-mTurquoise2

   Plasmid

   #136100

   Purpose

   FP for C-term fusion with CDS12 without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CTAG_AlaSerGly10xAlaGlySer_mTurquoise2
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. CDS_GUS

   Plasmid

   #136084

   Purpose

   For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS_GUS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. CDS12_eGFP-linker

   Plasmid

   #136086

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_eGFP(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDS12_mScarletI-linker

   Plasmid

   #136088

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_mScarletI(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMK110

   Plasmid

   #99535

   Purpose

   contains bgl operon sequence for E. coli in vitro transcription template

   Depositor

   Robert Landick

   Article

   Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

   Insert

   E. coli bgl antisense region containing bglG
   

   Tags

   None

   Promoter

   lambda Pr promoter used for in vitro transcriptio…

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046802

   Plasmid

   #145683

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCellFree_G03 PSMC1

   Plasmid

   #67087

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PSMC1 (PSMC1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   E275G, I287V and R294G

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-5X-1-DJ1-D149A

    Plasmid

    #29351

    Depositor

    Mark Cookson

    Article

    Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

    Insert

    DJ1 (PARK7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Asp149Ala

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. FT1

    Plasmid

    #31435

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Fused Toes Homolog (AKTIP Human)
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Syn3D33A

    Plasmid

    #13400

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. D33 of s…

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-FKBP12-C1-DH

    Plasmid

    #67901

    Purpose

    Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-FKBP12-DH
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Syn3E34A

    Plasmid

    #13401

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. E34 of s…

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pFastBac K-Flag M33

    Plasmid

    #1992

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

16. psicheck-2-Sfxn3

    Plasmid

    #48169

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of SFXN3 (SFXN3 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pJZ001

    Plasmid

    #120230

    Purpose

    Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    intB13 integrase casette
    

    Expression

    Bacterial

    Mutation

    bla-g717a (amp resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET:CCCT:GFP

    Plasmid

    #60738

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS314-MDM2

    Plasmid

    #18654

    Depositor

    Bert Vogelstein

    Article

    Oliner et al Nature. 1993 Apr 29. 362(6423):857-60.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    Gal1 promoter

    Expression

    Yeast

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pJKW1847

    Plasmid

    #174004

    Purpose

    Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

    Depositor

    Jing-Ke Weng

    Article

    Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

    Inserts

    PpCYP94D108
    ATR1 (ATR1 Mustard Weed)
    

    Expression

    Yeast

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX-5X-1-DJ1-M26I

    Plasmid

    #29353

    Depositor

    Mark Cookson

    Article

    Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

    Insert

    DJ1 (PARK7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Met26Ile

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

23. pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT

    Plasmid

    #24606

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, Y459A

    Available Since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLMB708

    Plasmid

    #83782

    Purpose

    Tartrate biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL0996 containing all of RL0997
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLMB710

    Plasmid

    #83783

    Purpose

    Formate biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL4393 (fdsG) containing all of RL4394
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLMB712

    Plasmid

    #83784

    Purpose

    Hesperetin biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLMB714

    Plasmid

    #83786

    Purpose

    C4-dicarboxylates biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL3424 (dctA)
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLMB705

    Plasmid

    #83787

    Purpose

    Mannitol biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL4218 (mtlB) containing all of RL4219
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLMB717

    Plasmid

    #83788

    Purpose

    Erythritol biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream pRL90085 containing all of pRL90087
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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