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pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD

Plasmid

#139113

Purpose

express prion like domain of hnRNPF

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

365-415

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAmreB

Plasmid

#149595

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAftsI

Plasmid

#149591

Purpose

all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMJB1-RPL1B-Cl

Plasmid

#160430

Purpose

Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

RPL1B

Tags

mCherry

Expression

Yeast

Mutation

Removed introns, shuffled codons to vary nucleoti…

Promoter

S. cerevisiae GAL1

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-FOXA1

Plasmid

#139304

Purpose

Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against FOXA1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND4_L17_S24_S11_S19_S13

Plasmid

#132732

Purpose

T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND4_L17_S24_S11_S19_S13

Expression

Bacterial

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAG_Ptet*_ToeRep_N01_switch

Plasmid

#132737

Purpose

Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_ToeRep_N01_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND3_L11_S11_S13_S19

Plasmid

#132731

Purpose

T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND3_L11_S11_S13_S19

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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