We narrowed to 7,147 results for: Trac;

1. pcDNA3.1-Beta3

   Plasmid

   #140988

   Purpose

   Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GBeta3 (GNB3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO-FOSL1

   Plasmid

   #170687

   Purpose

   doxycycline-inducible overexpression of FOSL1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-GRET

   Plasmid

   #158222

   Purpose

   Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   GFP-Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

   Plasmid

   #178528

   Purpose

   Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   rtTA, SspB-mScarlet-I-MYPT (1-169aa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, Tet responsive element

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GGamma1-GFP2

   Plasmid

   #140989

   Purpose

   Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma1-GFP2 (GNGT1 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Beta2

   Plasmid

   #166772

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB2 (GNB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Beta4

   Plasmid

   #166773

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB4 (GNB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ch-TOGKDP-GFP

   Plasmid

   #69112

   Purpose

   For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Silent mutations to confer shRNA resistance.

   Promoter

   CMV

   Available since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSIIpuro-Necl5-ScNeo

   Plasmid

   #209901

   Purpose

   To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   Necl5-ScNeo (Pvr Mouse)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSIIpuro-TGFa-ScNeo

    Plasmid

    #209895

    Purpose

    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-ScNeo (TGFA Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK CA ROCK2

    Plasmid

    #84649

    Purpose

    Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

    Insert

    ROCK2 (ROCK2 Bovine)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Constitutive Active (please see depositor comment…

    Promoter

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-GGamma8-GFP2

    Plasmid

    #140990

    Purpose

    Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma8-GFP2 (GNG8 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1-HsPcdh21-Myc-Flag-His

    Plasmid

    #210818

    Purpose

    Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Protocadherin 21 (CDHR1 Human)
    

    Use

    Tags

    Myc, 3x Flag, 10x His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xFlag-tdMCP-Erk2 (pErk reporter)

    Plasmid

    #226167

    Purpose

    Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-Erk2 (MAPK1 Human)
    

    Use

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. WIW_Scaffold2

    Plasmid

    #205574

    Purpose

    Nanobody that inhibits seeding of tau fibrils

    Depositor

    David Eisenberg

    Article

    Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    WIW_Scaffold2
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. (attB) BRaf with MS2-circRNA

    Plasmid

    #226164

    Purpose

    Used to integrate HA-tagged BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    2xHA-BRaf (BRAF Human)
    IRES-mCherry-P2A-PuroR
    MS2-circRNA barcode
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Syn-NS1

    Plasmid

    #175276

    Purpose

    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Tags

    NLS-dTomato (P2A cleavage)

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 TAP DHX36

    Plasmid

    #53547

    Purpose

    mammalian expression of DHX36

    Depositor

    Kathleen Collins

    Article

    Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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