We narrowed to 3,800 results for: golden gate

1. pXTK001 [Marker_KanR-NeoR-nptII_PartType2]

   Plasmid

   #229238

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_KanR-NeoR-nptII_PartType2

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   KanR-NeoR-nptII
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXTK007 [Marker_KanR-NeoR-neo_PartType4]

   Plasmid

   #229244

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_KanR-NeoR-neo_PartType4

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   KanR-NeoR-neo
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXTK008 [Marker_KanR-NeoR-nptII_PartType4]

   Plasmid

   #229245

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_KanR-NeoR-nptII_PartType4

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   KanR-NeoR-nptII
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL3342 PB-PEmax-puro

   Plasmid

   #226674

   Purpose

   PiggyBac cargo vector encoding PEmax and puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   PEmax
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

   Plasmid

   #229800

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHW1643

   Plasmid

   #229876

   Purpose

   gene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC
   

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHW1221

   Plasmid

   #229873

   Purpose

   gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   QF-act-4 3' UTR::SEC
   

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHW1177

   Plasmid

   #229872

   Purpose

   gene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   Tet-Off[TetR-QFAD]-act-4 3'UTR::SEC
   

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. dSpyCas9-E566-GS-LOV2-VPR

   Plasmid

   #231576

   Purpose

   Potent light-deactivated CRISPRa

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   dSpyCas9-VPR with an AsLOV2 insertion behind E566
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL3328 PB-sfGFP-WPRE-target

    Plasmid

    #226676

    Purpose

    PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    sfGFP-WPRE-site3target
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

11. CROPseq-Puro-2xMS2

    Plasmid

    #212627

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-Puro-12xMS2

    Plasmid

    #212628

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROPseq-Puro-4xMS2

    Plasmid

    #212626

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTBL3401 BtoApegRNA-sfGFP

    Plasmid

    #226667

    Purpose

    PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    BtoA pegRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB53

    Plasmid

    #226309

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
    

    Expression

    Bacterial

    Mutation

    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJB1559

    Plasmid

    #218590

    Purpose

    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TL1 alphaDdCBE T2 (DddA6-N)

    Plasmid

    #221995

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATP6 alphaDdCBE G2

    Plasmid

    #221959

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A, See depositor comments

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3'-com-gRNA GG backbone (MOBE1-4)

    Plasmid

    #219947

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-com-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge