We narrowed to 15,088 results for: pET

1. pET28b-ULP1-405-621

   Plasmid

   #229224

   Purpose

   For expression of ULP1 protein

   Depositor

   Andy LiWang

   Insert

   ULP1 (ULP1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   405-621 (truncated)

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET24a-VHH-tev-mCherry

   Plasmid

   #117752

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-6XHis-SUMO-SIRT2

   Plasmid

   #102622

   Purpose

   Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Tags

   6XHis-SUMO

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-37 and 357-389

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28sumo-hRig-I-Full-length

   Plasmid

   #210827

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

   Insert

   hRIG-I (DDX58) Full length protein (RIGI Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-6xHis-(pos36)GFP

   Plasmid

   #62937

   Purpose

   Expression of 6xHis-(+36)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (pos36)GFP
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMG046 pETARA-CK1α-His

   Plasmid

   #154074

   Purpose

   Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Tags

   GST and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-15b-UbcH5b/2419

   Plasmid

   #11475

   Depositor

   Allan Weissman

   Article

   Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

   Insert

   UbcH5b (UBE2D2 Human)
   

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET151-GST-PP-RPN13 (19-132)

   Plasmid

   #73742

   Purpose

   Expression of human RPN13 PRU domain in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PRU domain only (residues 19-132)

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-15b-TEV-C9R

    Plasmid

    #118754

    Purpose

    TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

    Depositor

    Yutaka Kuroda

    Article

    Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

    Insert

    Tobacco Etch Virus protease (NEWENTRY Synthetic)
    

    Tags

    C9R and His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid

    #107292

    Purpose

    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-GFP (SNCA Human)
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt, Kathleen Burns

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-NlaCascade-NLS-6xHis

    Plasmid

    #180213

    Purpose

    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas5-Cas8-Cas7
    

    Tags

    6XHis and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-His-SCov2-nsp7

    Plasmid

    #154757

    Purpose

    Expression of SARS-CoV-2 nsp7

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-His-SCov2-nsp8

    Plasmid

    #154758

    Purpose

    Expression of SARS-CoV-2 nsp8

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PET-eSpCas9-NLS-6xHis

    Plasmid

    #207379

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-EIN-FTO(32-505)

    Plasmid

    #186466

    Purpose

    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    Fat mass obesity associated protein (FTO Human)
    

    Tags

    6 Histidine-EIN (N-terminal domain of E.coli Enzy…

    Expression

    Bacterial

    Mutation

    amino acid 32-505

    Promoter

    T7

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge