We narrowed to 14,583 results for: pET

1. pET28a-SARS-CoV-2 NSP13

   Plasmid

   #176492

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

   Depositor

   Xiaodong Cheng

   Article

   White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

   Insert

   SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   I570 from SARS-CoV NSP13 was mutated to V to conv…

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pET22b-mEB1-eGFP-6xHis

   Plasmid

   #130981

   Purpose

   Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB1-GFP-6xHis (Mapre1 Mouse)
   

   Use

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMG046 pETARA-CK1α-His

   Plasmid

   #154074

   Purpose

   Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24a HAB1 (172-511)

   Plasmid

   #177844

   Purpose

   Bacterial Expression of HAB1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   Protein phosphatase 2C 16
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-his6-HA3-SNAPf

   Plasmid

   #188941

   Purpose

   T7 expression vector for making his6- and HA3-tagged SNAP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-SNAPf
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

   Plasmid

   #198750

   Purpose

   Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - SmallBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET_SZ1-RGG-GFP-RGG

   Plasmid

   #124937

   Purpose

   Expression of SZ1-RGG-GFP-RGG

   Depositor

   Benjamin Schuster, Matthew Good, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 3521 pET15b Bid p15

   Plasmid

   #8782

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p15 (Bid Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

9. PET-B-eSpCas9-NLS-6xHis

   Plasmid

   #207383

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, amino acids 1005-1013 repl…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET16b-Trx CC32/35SS

    Plasmid

    #36946

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    both active site cysteines (32,35) mutated to ser…

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Use

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET16b-Rnl2(1-249)K227Q

    Plasmid

    #14072

    Depositor

    Thomas Tuschl

    Insert

    Rnl2(1-249)
    

    Use

    Tags

    His (10x)

    Expression

    Bacterial

    Mutation

    changed Lys227 to Glutamine Gly112 is actually C…

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Thomas Wassmer, Zita Balklava

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET51b(+)_LAMA-F98 (for GFP)

    Plasmid

    #130714

    Purpose

    Plasmid encoding the bacterial expression of LAMA-F98 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-F98
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-His6-SUMO-MproC145A

    Plasmid

    #172653

    Purpose

    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

    Depositor

    Stephen Harrison

    Article

    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

    Insert

    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis Tag and SUMO

    Expression

    Bacterial

    Mutation

    Catalytic Cysteine 145 mutated to Alanine

    Promoter

    T7

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only