We narrowed to 24,466 results for: nes

1. pETSUMO2_Rhodoplanes-bGSDM

   Plasmid

   #212129

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Rhodoplanes bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. piGECI-NES-N1

   Plasmid

   #160422

   Purpose

   Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BirA*-HA-NES

   Plasmid

   #214473

   Purpose

   Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   BirA(R118G)
   

   Use

   Lentiviral; Gateway

   Tags

   HA tag and NES (Nuclear Exclusion Sequence)

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   EF-1a

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPlum-Farnesyl-5

   Plasmid

   #55968

   Purpose

   Localization: Plasma Membrane, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTFP1-Farnesyl-5

   Plasmid

   #55481

   Purpose

   Localization: Plasma Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. nes714tk/lacZ

   Plasmid

   #47614

   Purpose

   used to create transgenic mice expressing LacZ reporter with Nestin enhancer

   Depositor

   Urban Lendahl

   Article

   Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

   Insert

   nestin 2nd intron fragment (NES Human)
   

   Use

   Enhancer reporter

   Tags

   HSV tk promoter and lacZ

   Expression

   Mutation

   714 bp from 3' end of 2nd intron

   Promoter

   HSV tk promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pAS_MmaPylT_EF1_NES-MmaPylRS AF

   Plasmid

   #174902

   Purpose

   expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG and NES

   Expression

   Mammalian

   Mutation

   Y306A, Y384F

   Promoter

   EF1

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mWasabi-Farnesyl-5

   Plasmid

   #56503

   Purpose

   Localization: Plasma Membrane, Excitation: 493, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mWasabi

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. mCitrine-Farnesyl-5

   Plasmid

   #56558

   Purpose

   Localization: Membrane, Excitation: 516, Emission: 529

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. L_NES 67kDa

    Plasmid

    #201357

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Adeno_NES-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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