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pEGFPc1 H-RAS

Plasmid

#236449

Purpose

transient overexpression of H-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

H-RAS (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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mEos2-Farnesyl-5

Plasmid

#57375

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Catherine Galbraith, Michael Davidson

Article

Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

Insert

Farnesyl (HRAS Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Pan-Thyroid receptor scFv [N403/63]

Plasmid

#206751

Purpose

Mammalian Expression of Pan-Thyroid receptor scFV. Derived from hybridoma N403/63 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Thyroid receptor (Homo sapiens) recombinant scFV (THRA Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

tetO promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v3

Plasmid

#158030

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre stuffer v4

Plasmid

#158032

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCRISPR-Cre stuffer v4

Plasmid

#158033

Purpose

lenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA 3.1 BS (-) N protein

Plasmid

#188448

Purpose

SARS CoV-2 N protein expression

Depositor

Ajit Chande

Article

Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

Insert

SARS-CoV-2 N protein

Use

Tags

Strep-Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgUroD v4

Plasmid

#158036

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

UroD sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgItgb5 v3

Plasmid

#158038

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Itgb5 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-Cre sgRipk4 v3

Plasmid

#158035

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PLX306 iCre tetO NICD rtTA

Plasmid

#158049

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

mouse Notch Intra Cellular Domain

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

tetO promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-CreERT2 sgRipk4 v3

Plasmid

#158048

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-CreERT2 sgAdam10 v3

Plasmid

#158047

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJYDNg

Plasmid

#162452

Purpose

Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

Ha and myc and eUnaG2 fluorescent protein

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJYDNp

Plasmid

#162451

Purpose

Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2

Use

Shuttle vector bacteria/yeast

Tags

HA and myc

Expression

Mutation

Promoter

GAL1

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJYDC3

Plasmid

#162460

Purpose

Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…

Expression

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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