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Plasmid#170266PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1BDepositorArticleInsertnuclear export factor 1, B6 allele
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Nxf1C
Plasmid#170267PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1CDepositorArticleInsertnuclear export factor 1, CAST allele
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046638
Plasmid#153941PurposeYeast expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046622
Plasmid#153904PurposeYeast expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046635
Plasmid#149149PurposeYeast Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNX3a-PK12
Plasmid#78357Purposefission yeast epitope taggingDepositorInserts12xPK
kanMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCyc-Bem1
Plasmid#66573PurposeExpress an additional copy of Bem1, integrated at TRP locusDepositorInsertBem1
UseTagsExpressionYeastMutationF99I*(see below)PromoterpCyc1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sub2-3 in pRS314
Plasmid#90454PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-3 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-4 in pRS314
Plasmid#90455PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-4 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-6 in pRS314
Plasmid#90456PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-6 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-HIS3MX6
Plasmid#129100PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 upGFP
Plasmid#117899Purposeprotein expressionDepositorInsertAtCOPT1 upGFP (COPT1 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT6 upGFP
Plasmid#117903Purposeprotein expressionDepositorInsertAtCOPT6 upGFP (COPT6 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT2 upGFP
Plasmid#117901Purposeprotein expressionDepositorInsertAtCOPT2 upGFP (COPT2 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 uPIC
Plasmid#117898Purposeprotein expressionDepositorInsertAtCOPT1 uPIC (COPT1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCASB
Plasmid#190175PurposePlasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequenceDepositorInsertsgRNA cloning site
UseTagsExpressionBacterial and YeastMutationAdded 5'-agagacc-3' upstream and 5'…PromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCUP1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHis-AID*-9myc
Plasmid#99524PurposeC-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-GFP
Plasmid#99521PurposeC-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsGFPExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-6HA
Plasmid#99520PurposeC-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags6xHAExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKan-AID*-9myc
Plasmid#99522PurposeC-terminal AID*-9myc degron cassette with KanR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB6364
Plasmid#106151PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistanceDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-9myc
Plasmid#99518PurposeC-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNat-AID*-9myc
Plasmid#99523PurposeC-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-6FLAG
Plasmid#99519PurposeC-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags6xFLAGExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJS288
Plasmid#200714PurposeTo express yeast Hxt1 hexose transporter in yeastDepositorUseSynthetic BiologyTagsmCherryExpressionYeastMutationdomesticated for MoClo cloning (removal of intern…PromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only