Addgene

We narrowed to 2,294 results for: pET28

Showing: 701 - 750 of 2294 results

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pET28a LZ LED WT-FLAG

Plasmid

#199679

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED WT (GPR155 Human)

Use

Tags

6xHis and Flag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 em-blue-shift.2

Plasmid

#191901

Purpose

Encodes a GFP design, em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

em-blue-shift.2

Use

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN-MutTER

Plasmid

#178033

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

Changed T(108)A, E(109)A, R(110)A

Promoter

T7LacI

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-CI2F

Plasmid

#191254

Purpose

Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

Depositor

Arthur Laganowsky

Article

Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

Insert

Chymotrypsin Inhibitor 2

Use

Tags

Maltose Binding Protein

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-Pep-H

Plasmid

#185788

Purpose

Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-Pep4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-bhaB5

Plasmid

#167812

Purpose

Expresses His6- and MBP-tagged BhaB5 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB5

Use

Tags

His6, MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

Plasmid

#183387

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

redesigned Caspase-4 (AA94-377) (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

ANR261ENP, D315H, M318K, T352K

Promoter

T7

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)

Plasmid

#178034

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted

Promoter

T7LacI

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-CPL2/pET28a

Plasmid

#172095

Purpose

cDNA of CER11/CPL2 cloned into pET28a for protein expression

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G871L

Plasmid

#174798

Purpose

Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G871L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

G871L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A870L

Plasmid

#174797

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A870L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A870L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-K893I

Plasmid

#174799

Purpose

Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-K893I (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

K893I, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-XPO5-His6

Plasmid

#171562

Purpose

Encodes human XPO5 protein

Depositor

Yan Zeng

Insert

XPO5 (XPO5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head-LBD

Plasmid

#164837

Purpose

Expression of human talin2 head mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

Lipid binding deficient mutations

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-GFP-tglG

Plasmid

#134679

Purpose

Expresses His6-GFP-TglG fusion in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglG

Use

Tags

His6-GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a HAT epitope

Plasmid

#129445

Purpose

expression of HAT epitope

Depositor

Oskar Laur

Insert

HAT epitope

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF E281A

Plasmid

#139591

Purpose

expresses SznF E281A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E281A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 281 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF H225A

Plasmid

#139590

Purpose

expresses SznF H225A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H225A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed histidine 225 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF E215A

Plasmid

#139565

Purpose

expresses SznF E215A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E215A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-NT-G46D

Plasmid

#134588

Purpose

Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length (G154D)

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-FL-G84D

Plasmid

#134577

Purpose

Express human DDX43 mutant (full-length with G84D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-G84D (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length (G84D)

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-RupA_T1-NHis

Plasmid

#109259

Purpose

NRPS T domain with N-terminal 6His tag

Depositor

Emily Balskus

Article

Schneider et al Tetrahedron

Insert

RupA_T1

Use

Tags

His tag

Expression

Bacterial

Mutation

contains aa 917..1025 of WP_015523502.1

Promoter

T7

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-RupA_R-NHis

Plasmid

#109257

Purpose

NRPS R domain with N-terminal 6His tag

Depositor

Emily Balskus

Article

Schneider et al Tetrahedron

Insert

RupA_R

Use

Tags

His tag

Expression

Bacterial

Mutation

contains aa 2038..2443 of WP_015523502.1

Promoter

T7

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b+R3(R112C/T180C)C

Plasmid

#108194

Purpose

Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C)-P450cam fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b+3(R112C/T180C)C

Plasmid

#108193

Purpose

Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA3(R112C/T180C)-P450cam fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-Metavinculin tail

Plasmid

#107152

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

Insert

metavinculin (VCL Human)

Use

Tags

(His)8

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
csd5_41-192/pET28b(+)

Plasmid

#83296

Purpose

csd5, construct: 41-192, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1250

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-40

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd4_22-438/pET28b(+)

Plasmid

#83295

Purpose

csd4, construct: 22-438, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1075

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-21

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
csd2_121-308/pET28b(+)

Plasmid

#83292

Purpose

csd2, construct: 121-308, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1544

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-120

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
csd1_125-312/pET28b(+)

Plasmid

#83290

Purpose

csd1, construct: 125-312, pET28b(+) NC-tag

Depositor

SeWon Suh

Article

An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

Insert

hp1543

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-124

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (Cow 1-82)

Plasmid

#62062

Purpose

Expression of Cow p53 (residues 1-82) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-82

Promoter

T7

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (K24N)

Plasmid

#62079

Purpose

Expression of human p53 (1-73) (K24N) in e. coli

Depositor

Gary Daughdrill

Article

Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-73; K24N

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (Rabbit 1-87)

Plasmid

#62058

Purpose

Expression of Rabbit p53 (residues 1-87) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-87

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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