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Showing: 281 - 300 of 380 results

  1. pST50Trc3-CBPNDHFR

    Plasmid
    #63977
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63977-plasmid-map-sequence-id-177383

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  2. pST50Trc2-STRHISNDHFR

    Plasmid
    #63964
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63964-plasmid-map-sequence-id-177370

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  3. pST50Trc2-CBPHISNDHFR

    Plasmid
    #63963
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & Calmodulin Binding Peptide (CBP); TEV…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63963-plasmid-map-sequence-id-177494

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  5. pAd5-B6/7deltaE3

    Plasmid
    #175748
    Purpose
    A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.
    Depositor
    Fred Bunz
    Article
    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do
    Insert
    Adenovirus 5 genomic region 25043-35938
    Use
    Adenoviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Adenovirus sequences 27859-30803 replaced with Ba…
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    175748-plasmid-map-sequence-id-345607

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  7. pMT440

    Plasmid
    #8609
    Depositor
    Robert Hartley
    Article
    Yazynin et al Gene 1996 Feb 22;169(1):131-2.
    Insert
    barnase, barstar
    Use
    Tags
    as pMT416
    Expression
    Bacterial
    Mutation
    V36 codon of barnase of pMT416 replaced by polyli…
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  8. Xlim-1 (Lhx1) XH32-3

    Plasmid
    #74135
    Purpose
    Basic cDNA clone for the Xlim-1/Lhx1 gene. In Bluescript BS KS+. To prepare RNA for Sense: BamHI/T3; for antisense: XhoI/T7
    Depositor
    Igor Dawid
    Article
    Taira et al Genes Dev. 1992 Mar;6(3):356-66.
    Insert
    lhx1 (lhx1 Frog)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. Xlim-3 (lhx3)

    Plasmid
    #74139
    Purpose
    Partial cDNA clone for Xlim-3 (lhx3), named LH23-1. Cloned in BS KS+. To prepare RNA, sense: HindIII/T7; antisense: BamHI/T3.
    Depositor
    Igor Dawid
    Article
    Taira et al Dev Biol. 1993 Sep;159(1):245-56.
    Insert
    lhx3 (lhx3 Frog)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pCVD437

    Plasmid
    #63758
    Purpose
    7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  19. pILGFP10CF71

    Plasmid
    #185860
    Purpose
    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185860-plasmid-map-sequence-id-370799

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Showing: 281 - 300 of 380 results