-
Plasmid#96947PurposeExpresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsEGFP and dL5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PH-dL5**
Plasmid#96946PurposePLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsdL5 and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ MBIC5-mCer3
Plasmid#74116PurposeFor generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)DepositorInsertMBIC5-mCer3
UseTransgenic constructTagsmCerulean 3ExpressionMutationPromoterMyl7 (Cmlc2)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB2
Plasmid#168801PurposeFluorogen activating protein (FAP) DiB2DepositorInsertFluorogen activating protein DiB2
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB1
Plasmid#168800PurposeFluorogen activating protein (FAP) DiB1DepositorInsertFluorogen activating protein DiB1
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB3
Plasmid#168802PurposeFluorogen activating protein (FAP) DiB3DepositorInsertFluorogen activating protein DiB3
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-TagBFP-DiB-RM
Plasmid#168782PurposeActin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RMDepositorInsertLifeAct-TagBFP-DiB-RM
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_4
Plasmid#103922PurposeProtein expression and purification of PTN13 PDZ4 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-TEVsite-human Glomulin
Plasmid#52292Purposebacterial expression of human GlomulinDepositorInsertGlomulin (GLMN Human)
UseTagsGST and TEVExpressionBacterialMutationPromotertacAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_1
Plasmid#103919PurposeProtein expression and purification of PTN13 PDZ1 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_3
Plasmid#103921PurposeProtein expression and purification of PTN13 PDZ3 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_2
Plasmid#103920PurposeProtein expression and purification of PTN13 PDZ2 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_5
Plasmid#103923PurposeProtein expression and purification of PTN13 PDZ5 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-nLac
Plasmid#53126PurposeExpression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman GFAPAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSYC-176
Plasmid#178961PurposeExpresses human GFAP fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-190
Plasmid#178962PurposeExpresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationR79CPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfa28-nLac
Plasmid#53130PurposeExpression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains the A and B regions (bp -1757 to -1489) …Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfaABC1D-nLac
Plasmid#53131Purposeexpression of lacZ driven by human GfaABC1D promoterDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains ABC1D regions of Gfa2 promoter, -1757 to…Promoterhuman Gfa2Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only