Addgene

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FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-RatLC3

Plasmid

#55095

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-hLC3ΔG

Plasmid

#24986

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

deleted Gly at 121

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A G120

Plasmid

#123071

Purpose

Gateway entry clone encoding human MAP1LC3A G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acid 121. Stop codon after G120.

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK581] pRK5 HA-SLC38A9N

Plasmid

#136141

Purpose

mammalian expression of SLC38A9 N term fragment (1-119)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 G120

Plasmid

#123077

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 G120A

Plasmid

#123076

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120

Plasmid

#123074

Purpose

Gateway entry clone encoding human MAP1LC3B G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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SLC30A10-delta105-107

Plasmid

#65205

Purpose

Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

delta 105-107 amino acids

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T1_GST-LC3A

Plasmid

#223726

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

LC3A (MAP1LC3A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-120 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3C

Plasmid

#223727

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

LC3C (MAP1LC3C Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-126 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Aquamarine-LC3B G120A

Plasmid

#228563

Purpose

Encodes a donor-only, Aquamarine-tagged version of the inactive LC3B biosensor under the control of the CMV promoter

Depositor

Giulia Bertolin

Article

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Tags

Aquamarine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-LC3

Plasmid

#221738

Purpose

Stable expression of mRuby3-LC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LC3B (Map1lc3b Rat)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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SNAP-LC3 HRD

Plasmid

#207545

Purpose

Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences

Use

Tags

SNAPtag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-LC3B HRD

Plasmid

#207539

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human LC3B locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-Mch-MAP1LC3B

Plasmid

#201924

Purpose

For lentiviral transduction of Mch-MAP1LC3B

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-LC3B

Plasmid

#204573

Purpose

Expresses Ash-tagged LC3B in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

delta121-125aa

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX FLAG-SLC38A9.1

Plasmid

#199749

Purpose

Lentiviral construct to stably express SLC38A91.1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

SLC38A9.1

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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