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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,926 results for: NOL

Showing: 281 - 300 of 14926 results

  1. GGE254

    Plasmid
    #120789
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Lip-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120789-plasmid-map-sequence-id-263222

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  2. GGE255

    Plasmid
    #120788
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Lip-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120788-plasmid-map-sequence-id-310818

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  7. GGE207

    Plasmid
    #120792
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown-Mfe
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120792-plasmid-map-sequence-id-263227

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  14. pSTAP-seq_fly-ctrl

    Plasmid
    #86380
    Purpose
    Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Type
    Empty backbone
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86380-plasmid-map-sequence-id-173941

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  15. pGL3_zfh1_CP-candidate_luc+

    Plasmid
    #86391
    Purpose
    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  18. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  20. pSTAP-seq_fly-zfh1

    Plasmid
    #86381
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86381-plasmid-map-sequence-id-173935

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Showing: 281 - 300 of 14926 results