We narrowed to 5,782 results for: RIK

1. p55-H2B-Dendra2

   Plasmid

   #80609

   Purpose

   Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

   Insert

   H2B-Dendra2 (H2BC21 Synthetic, Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR8/GW_TOPO_Cdk12_Untagged

   Plasmid

   #127176

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   None

   Expression

   Mutation

   Promoter

   None

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. aav-tnt-actn2-gfp

   Plasmid

   #165034

   Purpose

   AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

   Depositor

   William Pu

   Article

   Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

   Insert

   ACTN2 (Actn2 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sema7a-Fc-His

   Plasmid

   #72175

   Purpose

   Expresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema7a (Sema7a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Bmpr2Δ751-813aa

   Plasmid

   #163603

   Purpose

   Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The LIMK-binding region (751-813 aa) is deleted

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

   Plasmid

   #132685

   Purpose

   AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Carnosine dipeptidase 2 (Cndp2 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nrp2.3-AP-His

   Plasmid

   #71974

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.3 (Nrp2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

   Plasmid

   #117405

   Purpose

   SpyCas9 sgRNA 1 targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SpyCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-HLA-B57

   Plasmid

   #135516

   Purpose

   Mammalian expression of myc-tagged HLA-B*57:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*57:1 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Use

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCDF-mTET1

    Plasmid

    #81052

    Purpose

    bacterial expression of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid

    #113436

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-HLA-B15

    Plasmid

    #135513

    Purpose

    Mammalian expression of myc-tagged HLA-B*15:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*15:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe zeo HMGA2

    Plasmid

    #17411

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    HMGA2 (Hmga2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-mEB1-mCherry-6xHis

    Plasmid

    #130984

    Purpose

    Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-mCherry-6xHis (Mapre1 Mouse)
    

    Use

    Tags

    mCherry-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-Meis1

    Plasmid

    #131605

    Purpose

    expression of mouse Meis1 in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Meis1 (Meis1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pORANGE GFP-Syt7 KI

    Plasmid

    #131488

    Purpose

    Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Syt7, Syt7 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-Mff(1-61)-PP-GST

    Plasmid

    #73042

    Purpose

    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Mff(1-61) (Mff Mouse)
    

    Use

    Tags

    GST-tag and PreScission protease site

    Expression

    Bacterial

    Mutation

    Truncation containing amino acids 1-61

    Promoter

    T7

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Plxna2-AP-His

    Plasmid

    #71997

    Purpose

    Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna2 (Plxna2 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-myc-CCR5

    Plasmid

    #98948

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Nrp2.1-Fc-His

    Plasmid

    #72098

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.1 (Nrp2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFUW-tetO-Irf2

    Plasmid

    #139814

    Purpose

    doxycycline-inducible expression of mouse Irf2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf2 (Irf2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pORANGE GFP-Nlgn3 KI

    Plasmid

    #131501

    Purpose

    Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3F-Strep-mMALT1

    Plasmid

    #33315

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    Malt1 (Malt1 Mouse)
    

    Use

    Tags

    3xFLAG and StrepOne

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pET21-Drp1

    Plasmid

    #72927

    Purpose

    Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    6xHis-tag and PreScission Protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

    Plasmid

    #136514

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    Codon-optimized AcrIIA5
    

    Use

    Lentiviral

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOP22-5'UTR-BBa_B0034

    Plasmid

    #216626

    Purpose

    NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

    Depositor

    Henrike Niederholtmeyer

    Article

    Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

    Insert

    T7p-5'B0034-NanoLuc-3'TMV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOP01-UTC-7.0

    Plasmid

    #216625

    Purpose

    NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

    Depositor

    Henrike Niederholtmeyer

    Article

    Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

    Insert

    T7p-5'g10-NanoLuc-3'TMV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ntng2.b-AP-His

    Plasmid

    #71995

    Purpose

    Expresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntng2.b (Ntng2 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge