We narrowed to 5,111 results for: codon optimized

1. pBUE411

   Plasmid

   #62200

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   OsU3 and Ubi

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4398

   Plasmid

   #74042

   Purpose

   Expresses human codon-optimized LbCpf1 and Lb crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Lb crRNA
   LbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV and human U6

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-DIO-EF1α-mKate2-biPAC

   Plasmid

   #169127

   Purpose

   AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Insert

   mKate2-biPAC
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   codon optimized to mouse

   Promoter

   EF1a

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

   Plasmid

   #86419

   Purpose

   C-terminal PCR tagging module for yeast codon optimized split GFP1-10

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   GFP1-10
   

   Expression

   Yeast

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIP hIL2 co

   Plasmid

   #90513

   Purpose

   Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   IL2 (IL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-wtTnsAB

   Plasmid

   #234731

   Purpose

   Wild-type human-codon optimized PseTnsAB in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseTnsAB
   

   Expression

   Mammalian

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-gRNA

   Plasmid

   #158973

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   gRNA3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

   Plasmid

   #127362

   Purpose

   gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. NylCp2-TS_pET28a

   Plasmid

   #215421

   Purpose

   Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2-TS
   

   Expression

   Bacterial

   Mutation

   D122G/H130Y/D36A/E263Q

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. evoBxb1_IVT

    Plasmid

    #222341

    Purpose

    Plasmid for evolved Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Template for in vitro transcription

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid

    #127370

    Purpose

    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA24N-sso7d-ligase

    Plasmid

    #87745

    Purpose

    IPTG-inducible expression of sso7d-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and codon optimized sso7d (Sulfolobus solfa…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ heiCas9

    Plasmid

    #186929

    Purpose

    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

    Depositor

    Joachim Wittbrodt

    Article

    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

    Insert

    myc-flexible linker-oNLS-Cas9-oNLS
    

    Use

    CRISPR

    Tags

    myc-flexible linker-optimizedNLS and optimizedNLS

    Expression

    Mammalian

    Promoter

    sp6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-MH6-RspCas13d_RspCasWYL1

    Plasmid

    #108304

    Purpose

    Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Inserts

    RspCas13d
    RspWYL1
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBUN421

    Plasmid

    #62204

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    TaU3 and Ubi

    Available Since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid

    #127354

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. wtBxb1_IVT

    Plasmid

    #222340

    Purpose

    Plasmid for wild type Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wtBxb1
    

    Use

    Template for in vitro transcription

    Promoter

    T7 (inactivated)

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSH-EFIRES-P-AtAFB2

    Plasmid

    #129715

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    no

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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