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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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XIAP (pBS0852)

Plasmid

#185282

Purpose

For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

XIAP (XIAP Human)

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1 G511N

Plasmid

#163626

Purpose

Expresses DCLK1 G511N in E. coli

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Mutation

G511N

Promoter

sv40

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

Plasmid

#232122

Purpose

A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

Tcf4ΔN (Tcf4 Mouse)

Use

Gateway cloning

Tags

myc

Mutation

lacks aa 1-56

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-mCherry

Plasmid

#65283

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

Plasmid

#104097

Purpose

Expresses TREAT-PK co-localization control in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-FNLS(RA)

Plasmid

#112671

Purpose

CMV expression vector for BE3-FNLS construct (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Tags

3x FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus and D10A

Promoter

CMV

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Kaede

Plasmid

#219433

Purpose

AAV expression vector for Kaede

Depositor

Yoshiho Ikeuchi

Article

Martins-Costa et al Cell Stem Cell. 2024 Jun 6;31(6):866-885

Insert

Kaede

Use

AAV

Promoter

CAG

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-TWIST1

Plasmid

#215022

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265F2

Plasmid

#164722

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)NG-UGI

Use

CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Promoter

Ef1a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgNC.SPA

Plasmid

#169834

Purpose

Expresses a negative control sgRNA

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

safe harbor sgRNA

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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Nuc-mTurq2-Apollo-NADP+

Plasmid

#193302

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

SV40 NLS and mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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