We narrowed to 4,121 results for: LYC;

1. pJS699

   Plasmid

   #168779

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD (S betacoronavirus)
   

   Tags

   Cmyc

   Mutation

   N343Q

   Promoter

   GAL1

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe puro-HA-PIK3CA(H1047R;G320E)

   Plasmid

   #33254

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and glycine 32…

   Promoter

   SV40

   Available Since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET32a-Spike-403-505

   Plasmid

   #165095

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCMV 3xFLAG-LC3A G120A

   Plasmid

   #123090

   Purpose

   Expresses 3xFLAG-LC3A G120A in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV 3xFLAG-GABARAPL1 G116A

   Plasmid

   #123101

   Purpose

   Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 GABARAPL2 G116A

   Plasmid

   #123087

   Purpose

   Gateway entry clone encoding human GABARAPL2 G116A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Glycine 116 to Alanine

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-DNPH1

   Plasmid

   #203224

   Purpose

   For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   DNPH1 (DNPH1 Human)
   

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB1-PRD(703-868, P801G)-Strep

   Plasmid

   #164444

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

   Insert

   GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
   

   Tags

   GB1-His6-TEV and Strep tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Proline 801 to Glycine

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. P41-bio

    Plasmid

    #47739

    Purpose

    Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P41
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP-KIF5A-Rigor

    Plasmid

    #172202

    Purpose

    Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 235 to Alanine

    Promoter

    CMV

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV TRE3G BCL2L11 G156E Neo

    Plasmid

    #140748

    Purpose

    Inducibly expresses G156E mutant BIM in mammalian cells

    Depositor

    Scott Dixon

    Article

    Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

    Insert

    BCL2L11 (BCL2L11 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed Glycine 156 to Glutamate

    Promoter

    CMV TRE3G

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHEN-CoV2-nanobody-Ty1

    Plasmid

    #194588

    Purpose

    Bacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

    Insert

    Ty1 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmirGLO-Bmpr2-Δ17

    Plasmid

    #49380

    Purpose

    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.

    Depositor

    Martine Roussel

    Article

    Murphy et al Cancer Res. 2013 Oct 21.

    Insert

    Bmpr2 (BMPR2 Mouse, Human)
    

    Use

    Luciferase

    Mutation

    changed nucleotides AC to TG at bp 7347-7348

    Promoter

    Human phosphoglycerate kinase (PGK) promoter

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. RAMA-bio

    Plasmid

    #47786

    Purpose

    Expresses enzymatically monobiotinylated full-length RAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RAMA
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSP9-bio

    Plasmid

    #47740

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP9 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP9
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBT259_(pRosa26-G-tTA2)

    Plasmid

    #36878

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    diphteria toxin A
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. P12-bio

    Plasmid

    #47725

    Purpose

    Expresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P12
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CLAG3.2-bio

    Plasmid

    #47797

    Purpose

    Expresses enzymatically monobiotinylated full-length CLAG3.2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised CLAG3.2
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmirGLO-Bmpr2-Δ19

    Plasmid

    #49381

    Purpose

    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-19 binding site. To be used for 3'UTR assay.

    Depositor

    Martine Roussel

    Article

    Murphy et al Cancer Res. 2013 Oct 21.

    Insert

    Bmpr2 (BMPR2 Mouse, Human)
    

    Use

    Luciferase

    Mutation

    changed nucleotides TG to AC at bp 7344-7345

    Promoter

    Human phosphoglycerate kinase (PGK) promoter

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge