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pXPR_BRD003-sgRBFOX1-2098

Plasmid

#115452

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2098 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgRBFOX1-2083

Plasmid

#115451

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2083 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Plxnd1-AP-His

Plasmid

#72006

Purpose

Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnd1 (Plxnd1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb2(L)-AP-His

Plasmid

#72002

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPgk-NLS-BioID2-HA

Plasmid

#232031

Purpose

mammalian expression (murine Pgk promoter) of nuclear targeted BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Inserts

NLS
Pgk1 promoter (Pgk1 Mouse, amplified from expression plasmid)

Tags

NLS

Expression

Mammalian

Mutation

Pgk1 promoter variant

Promoter

murine Pgk1

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-Eea1[36-126]-mCherry

Plasmid

#246260

Purpose

Rab5 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Eea1[36-126]-mCherry (Eea1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-B-GRPA

Plasmid

#226222

Purpose

Human RPA34 tagged to GFP under a EF1alpha promotor for generating stable HeLa Kyoto GFP-RPA34 using the Flp-In recombination system

Depositor

Cristina Cardoso

Article

Pabba et al Elife. 2023 Oct 31;12:RP87572. doi: 10.7

Insert

RPA34 (RPA2 Human)

Expression

Mammalian

Promoter

EF1ALPHA

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-FYCO1[963-1206]-mCherry

Plasmid

#246264

Purpose

Rab7 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-FYCO1[963-1206]-mCherry (Fyco1 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-2xEzrin-BioID2-HA

Plasmid

#227681

Purpose

To over express 2x Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

2x Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shAtg7-EGFP

Plasmid

#227684

Purpose

Expresses EGFP and shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Atg7 shRNA (Atg7 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT to BioID-nDIC2-3xFlag

Plasmid

#232418

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT to BioID-cDIC2-3xFlag

Plasmid

#232416

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 (322-366)

Plasmid

#193038

Purpose

Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 322-366

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-CAPN7-OSF

Plasmid

#193041

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 L61D (1-165)

Plasmid

#193033

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L61D, Residues 1-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP4A 153-222

Plasmid

#108256

Purpose

Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP4A residues 153-222 (CHMP4A Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Contains nonnative Gly-Cys for fluorescent labeli…

Promoter

T7

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP6 145-201

Plasmid

#104597

Purpose

Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP6 residues 145-201 (CHMP6 Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP7 366-453

Plasmid

#104598

Purpose

Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP7 residues 366-453 (CHMP7 Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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