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pAAV-EF1a-DIO-ASAP3-WPRE

Plasmid

#132318

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

Plasmid

#159904

Purpose

Mutagenesis of Kcnn3

Depositor

Larry Zweifel

Insert

Kcnn3 (Kcnn3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

Plasmid

#179459

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV1

Insert

JEDI-2P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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DRH031_ AAV-SIO-nEF-WT-THR

Plasmid

#225090

Purpose

Cre-dependent expression of wild-type thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

DN-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DRH032_ AAV-SIO-nEF-DN-THR

Plasmid

#225091

Purpose

Cre-dependent expression of dominant-negative thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

WT-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-stGtACR2-FusionRed

Plasmid

#217975

Purpose

AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

Depositor

Edward Callaway

Article

Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

Insert

stGtACR2-FusionRed

Use

AAV

Tags

FusionRed

Promoter

EF1a

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-GCaMP6s-WPRE-pGHpA

Plasmid

#67526

Purpose

"Fluorescent reporter for calcium imaging"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

GCaMP6s

Use

AAV

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-T2A-mCherry

Plasmid

#50972

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Has Service

AAV8

Insert

Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)

Use

AAV

Tags

T2A-mCherry and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pattB-LSL-AAVR-F2A-spCas9

Plasmid

#202459

Purpose

The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

Depositor

Jan Carette

Article

Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

Insert

AAVR (AU040320 Mouse)

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

mCherry fusion

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

Plasmid

#35498

Purpose

AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a)

Plasmid

#79672

Purpose

Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP (Scn9a jellyfish, Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

Plasmid

#198514

Purpose

Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

Plasmid

#84480

Purpose

AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM8) PAGER(TF)

Plasmid

#229999

Purpose

Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Mutation

V360A/R361A on KORD

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hGfap-R-CaMP1.07-WPRE-SV40

Plasmid

#164140

Purpose

AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

Depositor

Hirokazu Hirai, Hajime Hirase

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

R-CaMP1.07

Use

AAV

Promoter

hGFAP

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

Plasmid

#165429

Purpose

Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

FHS-paCaMKII

Use

AAV

Tags

FHS(Flag, HisX6, Strep) tag

Expression

Mammalian

Promoter

CaMKII0.4

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

Plasmid

#130990

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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