We narrowed to 8,294 results for: construct

1. p3E-CMVmin:GFP no-pA

   Plasmid

   #80819

   Purpose

   CMVmin-promoted GFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-GFP no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-GFPmem no-pA

   Plasmid

   #80817

   Purpose

   GFPmem without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   GFPcaax no pA p3E
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFP

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet-PcPT-ximD-ximE

   Plasmid

   #172654

   Purpose

   Production of decursinol in E. coli

   Depositor

   Minjuan Xu

   Article

   Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

   Insert

   PcPT, XimD and XimE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-EF1a-OCT4

   Plasmid

   #35391

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TALE-Oct4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pGBW-m4046574

   Plasmid

   #148976

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTH848-gst-disec

   Plasmid

   #89589

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-dis-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-mKate2 no stop

   Plasmid

   #80806

   Purpose

   red fluorophore without stop codon

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   pME-mKate2 no stop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCGN R-Ras 41A

   Plasmid

   #14730

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   41A

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pNH053

   Plasmid

   #42159

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mCherry-RT-mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEnt L1L3 tTA-2

    Plasmid

    #24414

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAS_gpyrG1

    Plasmid

    #90276

    Purpose

    (also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    gRNA (pyrg1)
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEnt R3L2-MCS

    Plasmid

    #26799

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    multiple cloning sequence
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-IRES-puro-polyA

    Plasmid

    #43967

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    puroR
    

    Use

    Gateway dest vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNH042

    Plasmid

    #42154

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNH041

    Plasmid

    #42153

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-mKate2-myc no-pA

    Plasmid

    #80812

    Purpose

    myc epitope fused to C-terminus of mKate2 without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mKateHA p3E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR mCherry L1-R5

    Plasmid

    #43963

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    mCherry
    

    Use

    Multisite gateway entr clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB3C5-proA-B0032-E0051

    Plasmid

    #107244

    Purpose

    Expresses lacZa-GFP fusion from constitutive proA promoter

    Depositor

    Joseph Davis, Robert Sauer

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProA-RBS(B0032)-Gemini (E0051)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    proA

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK371

    Plasmid

    #73596

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Point mutants in FAD2-3 ORF relative to deposited…

    Promoter

    GAL1 and GAL10

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046286

    Plasmid

    #149147

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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