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We narrowed to 9,075 results for: sgRNA

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Showing: 3021 - 3040 of 9075 results

  2. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    204956-plasmid-map-sequence-id-415598

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  4. pAC153-dual-dCas9VP96-sgExpression

    Plasmid
    #48239
    Purpose
    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA-Tag, HA-tag, and VP96
    Expression
    Mammalian
    Mutation
    D10A H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48239-plasmid-map-sequence-id-69546

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  6. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  8. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid
    #220493
    Purpose
    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.
    Depositor
    Sébastien Britton
    Publication
    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    220493-plasmid-map-sequence-id-437553

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  9. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Publication
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138525-plasmid-map-sequence-id-292292

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  13. pNB-Rep

    Plasmid
    #238996
    Purpose
    Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Publication
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    238996-plasmid-map-sequence-id-495027

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  20. pEPOR2KN0055

    Plasmid
    #117622
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
    Expression
    Plant
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    117622-plasmid-map-sequence-id-246075

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Showing: 3021 - 3040 of 9075 results