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pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2/25Q

Plasmid

#1384

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (25Q) (HTT Human)

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al bioRxiv 2022.10.04.510646

Insert

alpha synuclein (SNCA Human)

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Vcp

Plasmid

#123600

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

valosin containing protein (Vcp Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINB-hRNF216

Plasmid

#167354

Purpose

Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

Depositor

Bernhard Lechtenberg

Article

Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

Insert

E3 ubiquitin-protein ligase RNF216 (RNF216 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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VN-Sph1

Plasmid

#89755

Purpose

expresses human Synphilin fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

Insert

SNCAIP (SNCAIP Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-AICD

Plasmid

#69925

Purpose

mammalian expression of human AICD fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Tags

EGFP

Expression

Mammalian

Mutation

47 aa long

Promoter

CMV

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_RRMs

Plasmid

#157864

Purpose

express His tagged hnRNPA2 RRMs

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His

Expression

Bacterial

Mutation

190stop

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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