We narrowed to 30,195 results for: REP

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pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA

Plasmid

#220225

Purpose

AAV-STARR-seq validation vector containing VS33 enhancer

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Insert

Enhancer (VS33)

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-mPGK-NeoR-SV40 polyA-Z

Plasmid

#218909

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-Neomycine-SV40 polyA

Expression

Mammalian

Mutation

395T>C

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-mPGK-PuroR-SV40 polyA-Z

Plasmid

#218908

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-PuroR-SV40 polyA

Expression

Mammalian

Mutation

297T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-BlastR-Z

Plasmid

#218907

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-BlastR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-NeoR-Z

Plasmid

#218906

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-NeoR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-HygroR-Z

Plasmid

#218905

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-HygroR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-mPGK-BlastR-SV40 polyA-Z

Plasmid

#218903

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

mPGK-BlastR-SV40 polyA

Expression

Mammalian

Mutation

2468T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 +KTS C terminal HA

Plasmid

#217640

Purpose

Entry vector with WT1 +KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 +KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 -KTS C terminal HA

Plasmid

#217642

Purpose

Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Mutation

p.408_410delKTS

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

Plasmid

#212113

Purpose

Expression of GABARAP split tandem protein with mCherry

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mCherry-GABARAP (GABARAP Human, Discosoma sp.)

Tags

mCherry

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP

Plasmid

#212106

Purpose

Expression of GABARAP split tandem protein with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

Plasmid

#212109

Purpose

Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5

Plasmid

#166163

Purpose

Gateway entry vector for N. furzeriDDX5

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLC025 - pL0_StopCodon (CT)

Plasmid

#203905

Purpose

MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

Stop codon

Use

Synthetic Biology

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN381 - pTRV2-CHLH

Plasmid

#203886

Purpose

Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CHLH fragment

Use

RNAi

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1243 - pL0_pT3O (pro + 5U)

Plasmid

#203891

Purpose

T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3O promoter and 5'UTR

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1198 - pL0_pD4H (pro + 5U)

Plasmid

#203894

Purpose

D4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

D4H promoter and 5'UTR

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB55 - pL0_CrGLK (CDS)

Plasmid

#203903

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA_F164R238

Plasmid

#216230

Purpose

S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed arginine 238 to serine, phenylalanine 168…

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_A467

Plasmid

#216234

Purpose

S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed alanine 467 to valine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_T522

Plasmid

#216235

Purpose

S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed threonine 522 to isoleucine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Blast

Plasmid

#214014

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Hygro

Plasmid

#214015

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-Blast-TNNT2-Zeo

Plasmid

#214018

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Parkin(C431N)-A92mKO2

Plasmid

#213545

Purpose

Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

C431N mutation in Parkin

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(R104A)-A92mKO2

Plasmid

#213556

Purpose

Lentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R104A mutation in Parkin

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-CMV(trunc)-Parkin-A92mKO2

Plasmid

#213549

Purpose

Lentiviral vector for stable low expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Truncated CMV

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(N273K)-A92mKO2

Plasmid

#213555

Purpose

Lentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N273K mutation in Parkin

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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