We narrowed to 5,782 results for: RIK

1. pEGFP-PTRF

   Plasmid

   #68401

   Purpose

   Expression of fluorescently tagged PTRF in mammalian cells

   Depositor

   Rob Parton

   Article

   Hill et al Cell. 2008 Jan 11;132(1):113-24. doi: 10

   Insert

   PTRF (Cavin1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Kif1A(1-489)-DHFR-myc

   Plasmid

   #117833

   Purpose

   Constitutively active Kinesin-3 motor tagged with DHFR and myc

   Depositor

   Thomas Schwarz

   Article

   Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

   Insert

   Kif1A (1-489) (Kif1a Mouse)
   

   Use

   Tags

   DHFR and myc

   Expression

   Mutation

   only aa 1-489 (motor domain)

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Susd4(3.7)-EGFP-sv40

   Plasmid

   #184413

   Purpose

   AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Susd4 gene

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA6-N-3XFLAG-Ubac2

   Plasmid

   #123597

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   ubiquitin associated domain containing 2 (Ubac2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

   Plasmid

   #127865

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   deleted the c-terminal 20 amino acids

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-mTDGa.1

   Plasmid

   #81051

   Purpose

   bacterial expression of catalytically dead murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   asparagine 151 changed to alanine

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HMGA2(3'UTR DEL)+GFP

   Plasmid

   #25406

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   high mobility group AT-Hook 2 (Hmga2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   3" UTR is absent

   Promoter

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

   Plasmid

   #113952

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S and S212N su…

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. F-MED28

   Plasmid

   #15368

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

   Insert

   MED28 (MED28 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

    Plasmid

    #127866

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    C-terminal 20 amino acids only

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTFP1-H1-10

    Plasmid

    #55487

    Purpose

    Localization: Nucleus/Histones, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    H1 (H1f0 Mouse)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-Cln6

    Plasmid

    #78109

    Purpose

    mammalian expression of mouse Cln6 with C terminal EGFP fusion

    Depositor

    Thomas Braulke

    Article

    Thelen et al PLoS One. 2012;7(4):e35493. doi: 10.1371

    Insert

    Cln6 (Cln6 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pASK-mSAN1-Streptag

    Plasmid

    #117165

    Purpose

    Expresses Strep-tagged murine SAN1 nuclease in bacteria

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    mSAN1 (Fam120b Mouse)
    

    Use

    Tags

    Streptag

    Expression

    Bacterial

    Mutation

    Promoter

    tetR/tetA

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

    Plasmid

    #105037

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Prom1 D-5

    Plasmid

    #184017

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Sema7a-AP-His

    Plasmid

    #72049

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. Mouse GFP-PRMT7 Wild Type

    Plasmid

    #65056

    Purpose

    Expresses wild type mouse GFP-PRMT7

    Depositor

    Steven Clarke

    Article

    Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

    Insert

    PRMT7 (Prmt7 Mouse)
    

    Use

    Bacterial propagation

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

22. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. Plxna1-AP-His

    Plasmid

    #71996

    Purpose

    Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna1 (Plxna1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. TFORF3454

    Plasmid

    #144930

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF653 (ZNF653 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAdTrack: shRev-Erbalpha

    Plasmid

    #22749

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Adenoviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pAAV-DYRK2-2A-GFP

    Plasmid

    #118284

    Purpose

    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV-GSDMD_NoCys-Myc

    Plasmid

    #201169

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    C39A, C57A, C77A, C122A, C265A, C192A

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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