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Plasmid#40384DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTags6xHisExpressionMutationaa1867-2161, C2024S/C2025SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus_Sumostar/LRRK2 1867-2161
Plasmid#40391DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pQdCas12a.luxR(mut)-sggfp(C)
Plasmid#236043PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-SARS-CoV-2_Spike_WT-VA
Plasmid#191216PurposeLentiviral expression of SARS-CoV-2 Spike_WT in mammalian cellsDepositorInsertSPIKE_SARS2_WT (S SARS CoV-2)
UseLentiviralTagsVA tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(B)
Plasmid#236041PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(A)
Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S17/43A)-TFAM-V5
Plasmid#202404Purposeexpresses MTS(S17/43A)-TFAM-V5 in mammalian cellsDepositorInsertTranscription Factor A, Mitochondrial (TFAM Human)
UseTagsV5ExpressionMammalianMutationS17A, S43APromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-hnRNPA2B1
Plasmid#181714PurposeGalactose inducible expression of hnRNPA2B1DepositorInserthnRNPA2B1 (HNRNPA2B1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_NRF2
Plasmid#214692PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human NRF2DepositorInsertdgRNA_NRF2 (NFE2L2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-PHOSPHO1
Plasmid#224896PurposeExpress N-terminal mCherry fused to human PHOSPHO1 in mammalian cellsDepositorUseTagsmCherryExpressionMammalianMutationstart codon is deletedPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro
Plasmid#216666PurposeExpresses tTa-inducible MyoD and miRFP703, with puromycin resistanceDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)
Plasmid#225437PurposeTranscriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.DepositorInsertCBS-att-OCS-BeYDVRep/RepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
Plasmid#225431PurposeOCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-att-OCSterm-att-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBB328
Plasmid#221533PurposePlasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.DepositorInsertsvioABCE
Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
Tetracycline antibiotic cassette from pRK415
UseTagsExpressionBacterialMutationPromoterNone, contains RBS siteAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLPC-C-SF-TAP-DEST-TLK1- Q479E
Plasmid#218830PurposeExpresses C-terminal Strep-FLAG-tagged Q479E human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 Q479E (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationQ479EPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~41-mEGFP(A206K)-TwStrep
Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-M337V
Plasmid#181721PurposeGalactose inducible expression of TDP-43-M337VDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationM337VPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-N267S
Plasmid#181720PurposeGalactose inducible expression of TDP-43-N267SDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationN267SPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-Q331K
Plasmid#181719PurposeGalactose inducible expression of TDP-43-Q331KDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationQ331KPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATAXIN3:1-361_BV
Plasmid#209732PurposeBacterial expression for structure determinationDepositorInsertAtaxin 3 (ATXN3 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterPolyhedrinAvailable sinceNov. 13, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Myc-Nix-S35A
Plasmid#197562PurposeMammalian expression of myc-tagged human Nix with an alanine substitution of serine-35. Based on Myc-Nix (#100795)DepositorInsertBCL2 interacting protein 3 like (BNIP3L Human)
UseTagsMycExpressionMammalianMutationSerine 35 changed to alaninePromoterCMVAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-PAG1-GFP
Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Pre…ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_67_pKD46.10
Plasmid#178942PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_37_pKD46.10
Plasmid#178927PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_26.10
Plasmid#178919PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGB-05-06-GST-mCherry-ATG14
Plasmid#187988PurposePlasmid for the protein expression of GST-mCherry-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1322DepositorInsertATG14 (ATG14 Human)
UseTagsGST-mCherryExpressionInsect and MammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_15a.10
Plasmid#178913PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
isoform PKC Theta (JCE782)
Plasmid#182026Purposeyeast expression of mouse PKC Theta with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Theta (Prkcq Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLuc VLP
Plasmid#182213PurposeYIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-GLuc
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_Rev7_1-211_SA112_KVK
Plasmid#105634PurposeExpresses mouse monomeric Rev7 proteinDepositorInsertRev7 (Mad2l2 Mouse)
UseTagsExpressionBacterialMutationMutations: F11S, G12A, V132K, C133V, A135KPromoterT7Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_67.10
Plasmid#178940PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_47_pKD46.10
Plasmid#178932PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_5a5a.10
Plasmid#178933PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits