We narrowed to 9,183 results for: try;

1. pENTR4_ELAVL1

   Plasmid

   #106103

   Purpose

   Entry vector for ELAVL1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   ELAVL1 (ELAVL1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. G1397 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157840

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSaPIPLC His

   Plasmid

   #173810

   Purpose

   Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Anne Gershenson, Mary F Roberts

   Article

   Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

   Insert

   Staphylococcus aureus plc
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR1A mKate2-hu_ncOGT WT

   Plasmid

   #154282

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   WT OGT

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ230-RRVQ

   Plasmid

   #200188

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RRVQ
   

   Use

   CRISPR; Gateway compatible lbcas12a-rrv entry clo…

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, D156R, R182V, E795Q

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2xFlag CMV2-YAP2-1st&2nd WW mutant

   Plasmid

   #19048

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Jul 17. ():.

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 changed to Alanine,and Proline 202…

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-YFP-DEPTOR

   Plasmid

   #73390

   Purpose

   Expresses HA and YFP tagged DEPTOR

   Depositor

   Taekjip Ha, Jie Chen

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pMX-hTAF11

   Plasmid

   #44341

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF11 (TAF11 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMX_hTAF5

   Plasmid

   #44336

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF5 (TAF5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ141-ZmUbi-RZ-Fn

    Plasmid

    #108864

    Purpose

    FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Type

    Empty backbone

    Use

    CRISPR; Gateway compatible crrna entry clone

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CYFIP2.mCherry

    Plasmid

    #122052

    Purpose

    Expresses human CYFIP2 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ145

    Plasmid

    #98405

    Purpose

    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYZ149

    Plasmid

    #98407

    Purpose

    Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
    downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC841 - pAAV minP secNLuc

    Plasmid

    #82498

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

    Plasmid

    #139445

    Purpose

    Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag E2-crimson
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid

    #127359

    Purpose

    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2

    Plasmid

    #62168

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ230-RVQ

    Plasmid

    #200187

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. KCC2B_HUMAN_D0

    Plasmid

    #79718

    Purpose

    This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2B
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only