We narrowed to 9,265 results for: yeast expression

1. p415-GalL-Cas9-CYC1t

   Plasmid

   #43804

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GalL

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVTU_260_BirA

   Plasmid

   #63682

   Purpose

   Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

   Insert

   BirA
   

   Use

   Tags

   6X Histidine and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   ADH1/yeast

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY2-MET-IV

   Plasmid

   #218117

   Purpose

   MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY2-LYS-IV

   Plasmid

   #218118

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV0757

   Plasmid

   #133509

   Purpose

   Expression of 3GFP tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSII42H-TDH3pr-mNeon

   Plasmid

   #194539

   Purpose

   High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

   Depositor

   Patrick Gibney

   Article

   Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

   Insert

   hphMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-His tagged nuclear Pif1

   Plasmid

   #65047

   Purpose

   expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

   Depositor

   Jean-Baptiste Boulé, Virgina Zakian

   Article

   Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

   Insert

   Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
   

   Use

   Tags

   histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH847-gst-optsc

   Plasmid

   #96908

   Purpose

   Plasmid for yeast expression of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   yeast codon optimised glutathione-S transferase
   

   Use

   Tags

   8xhistidine

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV0765

    Plasmid

    #133501

    Purpose

    Expression of mCherry targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTEF6-7

    Plasmid

    #124583

    Purpose

    Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV0532

    Plasmid

    #133502

    Purpose

    Expression of mTagBFP2 targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV0526

    Plasmid

    #133500

    Purpose

    Expression of sfGFP targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCUP1-DuDre-Atg8-404

    Plasmid

    #69201

    Purpose

    RFP-Atg8 (PAS marker) with improved property

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    DuDre-Atg8
    

    Use

    Tags

    DsRed Express 2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV0761

    Plasmid

    #133494

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LT2_14_GlyOx

    Plasmid

    #177711

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0620

    Plasmid

    #133506

    Purpose

    Expression of eGFP tagged PCNA to monitor DNA replication

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p413TEF1-ATR1(pYL573)

    Plasmid

    #178288

    Purpose

    Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    ATR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMM4

    Plasmid

    #170359

    Purpose

    Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PCYC1_SEC61_mCherry_LEU2_TCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PCYC1

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV0785

    Plasmid

    #133507

    Purpose

    Expression of mCherry tagged PCNA to monitor DNA replication

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge