We narrowed to 2,325 results for: PUC19

1. pMG

   Plasmid

   #138264

   Purpose

   Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Inserts

   eGFP flanked by iCas9 sites
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tef1

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITGFP89

   Plasmid

   #83560

   Purpose

   Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TEF1 promoter -yEGFP-CLN2PEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC-CRISPR1

   Plasmid

   #120366

   Purpose

   Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   CRISPR1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVC-CRISPR

   Plasmid

   #120369

   Purpose

   Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Inserts

   CRISPR1
   CRISPR2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pILGFP9E3

   Plasmid

   #185842

   Purpose

   Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pILGFP86

   Plasmid

   #83543

   Purpose

   Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   RPL4A promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPL4A

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTX1-EV71-SL2

   Plasmid

   #126043

   Purpose

   In-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   Enterovirus 71 5'UTR SL2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pADH118-2

   Plasmid

   #90984

   Purpose

   NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT9993-Hypa-ABE

   Plasmid

   #163001

   Purpose

   plasmid expressing Hypa-ABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   Hypa-ABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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