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tdEos-LaminB1-10

Plasmid

#57632

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

LaminB1 (LMNB1 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET:ACAT:GFP

Plasmid

#60740

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET:GCAT:GFP

Plasmid

#60736

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLYS1- DdMCU_AIIA, Cterm Flag

Plasmid

#253538

Purpose

Expresses C-terminal Flag tagged DdMCU_AIIA

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU (DDB_G0286429 Dictyostelium discoideum)

Use

Lentiviral

Tags

Flag

Mutation

changed D192A and E195

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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p5g-Opto_N449S

Plasmid

#253285

Purpose

Opto-CheY N449S expressed using the pro5 promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-PiTq

Plasmid

#248769

Purpose

Mammalian expression vector for PiTq

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2026 Mar 31;12

Insert

Pi-Tq

Use

AAV

Expression

Mammalian

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_N449S

Plasmid

#253256

Purpose

Opto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_V416T

Plasmid

#253257

Purpose

Opto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with V416T mutation

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pMB-pXD80-Eadh1

Plasmid

#249567

Purpose

Expresses Eadh1

Depositor

Emily Balskus

Article

Bae et al Proc Natl Acad Sci U S A. 2025 Dec 30;12

Insert

Eadh1

Tags

His6

Expression

Bacterial

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pHSP90(G97D)-mOFP

Plasmid

#228195

Purpose

expressed human Hsp90 (G97D mutation) with a mOFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP90AA1(G97D) (HSP90AA1 Human)

Tags

mOFP

Expression

Mammalian

Mutation

G97D mutation

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pJL VanS NarX Chimera

Plasmid

#225655

Purpose

Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

VanS-NarX

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Mutation

Transmembrane and sensing domain replaced to dete…

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-SOS

Plasmid

#208278

Purpose

Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-EGFR

Plasmid

#208280

Purpose

Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-EGFR

Use

Lentiviral

Mutation

H225N in mCherry- please see depositor comment

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-EGFR

Plasmid

#208281

Purpose

Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-EGFR

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJS124

Plasmid

#207593

Purpose

GFP-BIB H20A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB H20A

Expression

Bacterial

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ27

Plasmid

#207604

Purpose

GFP-BIB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ26

Plasmid

#207603

Purpose

GFP-AIA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJS119

Plasmid

#207592

Purpose

GFP-TonB deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS104

Plasmid

#207589

Purpose

GFP-TolA deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS70

Plasmid

#207586

Purpose

GFP-TolA H22A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA H22A

Expression

Bacterial

Mutation

H22A

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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