We narrowed to 10,458 results for: T7

1. 6BY9 (EHMT1)

   Plasmid

   #119956

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   EHMT1 (EHMT1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pPSU60

   Plasmid

   #173833

   Purpose

   Expresses 60 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   HSTPABPACRCC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-6xHis-GFP(0)

   Plasmid

   #199166

   Purpose

   used to express a neutral GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(0)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, I138R, D207R, N222K, L246R

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Promoter

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTETDuet_M42FG121VDHFR_R166QTS

   Plasmid

   #191298

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M42F/G121V DHFR, R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JMC044-B12

   Plasmid

   #58752

   Purpose

   bacterial expression of PDB 4NW3

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4NW3 (KMT2A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available since

   Aug. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pET-Sac-Abeta(MC1-40)

   Plasmid

   #127152

   Purpose

   Expresses N-terminal cysteine Abeta(1-40)

   Depositor

   James Nowick

   Insert

   Abeta(MC1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Reb_206 aka PAS629

   Plasmid

   #71234

   Purpose

   Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Use

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 6xHis-MBP-huBoCas12a

    Plasmid

    #174673

    Purpose

    Expresses 6xHis-MBP tagged BoCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBoCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJBL7004

    Plasmid

    #136943

    Purpose

    Malachite green aptamer, T7 promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    malachite green aptamer (MGA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pT7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEZY19

    Plasmid

    #18668

    Purpose

    pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSANG10-3F

    Plasmid

    #39264

    Depositor

    John McCafferty

    Article

    Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

    Type

    Empty backbone

    Use

    Bacterial expression, bacterial expression of re…

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Himar-dCas9

    Plasmid

    #137079

    Purpose

    Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PE-SUMO CRYBB1

    Plasmid

    #217675

    Purpose

    For expression of SUMO tagged human beta B1 crystallin

    Depositor

    Larry David

    Insert

    CRYBB1 (CRYBB1 Human)
    

    Use

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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