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pNBU2_erm_P1T_DP-GH023

Plasmid

#90317

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Publication

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3.1(+)-NEMOb

Plasmid

#189934

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Publication

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOb

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CE-8e-SpRY

Plasmid

#188913

Purpose

A-to-G base editor

Depositor

Xu Ma

Publication

Cao et al Mol Ther Nucleic Acids. 2022 May 4;28:73

Insert

nSpRYCas9 ecTadA-8e

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTHSSe_42

Plasmid

#109240

Purpose

pSC101 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV GATA2

Plasmid

#83820

Purpose

Mammalian expression of mouse Gata2

Depositor

Douglas Engel

Insert

GATA2 (Gata2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pGGD003

Plasmid

#48835

Purpose

Provides linker:mCherry as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

Linker-mCherry

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCJ189

Plasmid

#162666

Purpose

pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

Depositor

Gregg Beckham

Publication

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Insert

MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker

Tags

His

Expression

Bacterial

Mutation

codon optimized for expression in E. coli K12

Promoter

T7/lac

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-SIN-PTEN-Y138L

Plasmid

#30378

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y138L (PTEN Human)

Expression

Mammalian

Mutation

Y138L

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pIF1079

Plasmid

#237445

Purpose

Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

Depositor

IIya Finkelstein

Publication

Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103

Inserts

SpCas9
Efe1-RT
EMX1 sgRNA
Efe1-ncRNA targeting EMX1

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG, H1, and U6

Available since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAM552

Plasmid

#154131

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Publication

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

wt bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pBiFC-PBZ-VC

Plasmid

#110648

Purpose

expresses a PAR-binding (PBZ) domain fused to venus C-terminus

Depositor

Chris Lord

Publication

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

HA and VC

Expression

Mammalian

Promoter

CMV

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKL2310

Plasmid

#187875

Purpose

Empty pVS1-based INTEGRATE vector with loxP cargo

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBiFC-PBZ-VN

Plasmid

#110646

Purpose

expresses a PAR-binding (PBZ) domain fused to venus N-terminus

Depositor

Chris Lord

Publication

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

DDK and VN

Expression

Mammalian

Promoter

CMV

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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ChlorON-1

Plasmid

#193477

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Publication

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-1

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Publication

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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