We narrowed to 10,632 results for: t7

1. pETMCSI-T4P_Y320A

   Plasmid

   #122522

   Purpose

   Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

   Depositor

   Gottfried Otting

   Article

   Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

   Insert

   gp43 - DNA polymerase (43 Escherichia virus T4)
   

   Expression

   Bacterial

   Mutation

   tyrosine 320 changed to alanine

   Promoter

   T7 promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-MBP-huMb3Cas12a

   Plasmid

   #174677

   Purpose

   Expresses 6xHis-MBP tagged Mb3Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb3Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-MBP-huBsCas12a

   Plasmid

   #174671

   Purpose

   Expresses 6xHis-MBP tagged BsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PE-SUMO CRYBB1

   Plasmid

   #217675

   Purpose

   For expression of SUMO tagged human beta B1 crystallin

   Depositor

   Larry David

   Insert

   CRYBB1 (CRYBB1 Human)
   

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Reb_206 aka PAS629

   Plasmid

   #71234

   Purpose

   Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Himar-dCas9

   Plasmid

   #137079

   Purpose

   Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-gRNA

   Plasmid

   #46759

   Purpose

   gRNA cloning vector for in vitro transcription of target gRNA

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   gRNA core
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSU20

   Plasmid

   #173829

   Purpose

   Expresses 20 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACCBPv3
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJExpress414_GmPOPB

   Plasmid

   #92234

   Purpose

   Express wild type prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA2219

    Plasmid

    #38152

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

    Insert

    DsbC (dsbC E. coli)
    

    Tags

    N-terminal leader peptide (cloning artifact)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPSU50

    Plasmid

    #173832

    Purpose

    Expresses 50 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPSU100

    Plasmid

    #173835

    Purpose

    Expresses 100 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bQRS
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-KpAGO207-1251

    Plasmid

    #117854

    Purpose

    Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

    Depositor

    Kotaro Nakanishi, Dinshaw Patel

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Argonaute
    

    Tags

    6xHis-SUMO encoded in vector

    Expression

    Bacterial

    Mutation

    missing first 206 amino acid residues

    Promoter

    T7

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPSU30

    Plasmid

    #173830

    Purpose

    Expresses 30 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    HSTPABPACS100BCBP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPSU10

    Plasmid

    #173827

    Purpose

    Expresses 10 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML433

    Plasmid

    #177190

    Purpose

    Expresses recombinant HyPro enzyme in E. coli

    Depositor

    Eugene Makeyev

    Article

    Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

    Insert

    HyPro enzyme
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-mSA-EGFP

    Plasmid

    #39862

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin fused to EGFP
    

    Tags

    6xHis, EGFP, and FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAPK1

    Plasmid

    #73248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK1 (MAPK1 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. OmpX

    Plasmid

    #213136

    Purpose

    Expresses outer membrane protein X

    Depositor

    Sebastian Hiller

    Article

    Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

    Insert

    OmpX (24-171) (ompX E.coli (K12))
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUG-302

    Plasmid

    #104731

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC11_EL3
    

    Tags

    HIS-Ubq, HA-E2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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