We narrowed to 9,520 results for: try

1. pET21-pelB-LaRIgG-1

   Plasmid

   #228995

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-1
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-LaMIgG-5

   Plasmid

   #228991

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-5
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET51b-His-TEV-SNAP-tag_fast

   Plasmid

   #167271

   Purpose

   Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAPf-tag
   

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMX_mTBP

   Plasmid

   #44333

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TBP (Tbp Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET15b CnA CnB

   Plasmid

   #11787

   Depositor

   Anjana Rao

   Article

   Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

   Insert

   CnA CnB (PPP3R1, PPP3CA Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-AtMIR390a-B/c

   Plasmid

   #51778

   Purpose

   Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtMIR390a-B/c
   

   Use

   Gateway entry vector

   Mutation

   A. thaliana MIR390a precursor sequence including …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ265E2

   Plasmid

   #164719

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDarmo.CMVT_v1

   Plasmid

   #133072

   Purpose

   Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

   Depositor

   David Sabatini, Kacper Rogala

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   3C-Flag-TwinStrep and TwinStrep-Flag-3C

   Expression

   Mammalian

   Promoter

   CMVT (Enhanced CMV)

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223-EGFP

   Plasmid

   #209057

   Purpose

   Gateway-compatible entry vector, with insert of the EGFP gene

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET23 P53DBD

    Plasmid

    #174503

    Purpose

    express P53 DNA binding domain (94-312) in E.coli without tag

    Depositor

    Stewart Loh

    Article

    Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

    Insert

    P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRE-Tight-FLAG-CYLD-WT

    Plasmid

    #60030

    Purpose

    WT CYLD under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

13. HA-MAP1LC3C

    Plasmid

    #137758

    Purpose

    Expression of LC3C

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3C (MAP1LC3C Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR_L1-Turbo-YFP-NLS-STOP-L2

    Plasmid

    #127361

    Purpose

    gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mouse CENP-B-EGFP

    Plasmid

    #107264

    Purpose

    mouse CENP-B-EGFP in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    CENP-B-EGFP (Cenpb Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pM-mTagBFP2

    Plasmid

    #228615

    Purpose

    Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe-Puro-REDD1-HA

    Plasmid

    #133550

    Purpose

    Expresses HA tagged REDD1 in Mammalian cells

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-CYLD-STOP

    Plasmid

    #60077

    Purpose

    N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. neurod1:cre; cmlc2:EGFP

    Plasmid

    #173480

    Purpose

    cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Cre
    

    Use

    Zebrafish expression

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDD2148

    Plasmid

    #153968

    Purpose

    pCMV6-Entry vector encoding human IRAK1 protein 196F

    Depositor

    Dirk Dittmer

    Article

    Seltzer et al J Virol. 2020 May 4;94(10). pii: JVI.021

    Insert

    IRAK1 (IRAK1 Human)
    

    Tags

    Myc-DDK

    Expression

    Mammalian

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge