We narrowed to 26,293 results for: STI

1. 564aa ELL2 bluescript

   Plasmid

   #127267

   Purpose

   For in vitro translation of human ELL2 lacking COOH region

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Addgene Plasmid 127256--SphI site filled in, then…

   Promoter

   T7

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX304 PCSK5 (H516P)-V5 blast

   Plasmid

   #83103

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Histidine 516 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. csd6_17-330/pET28b(+) N-tag

   Plasmid

   #71353

   Purpose

   csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 )
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

4. csd6_13-330_E110A/pET28b(+) N-tag

   Plasmid

   #71354

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Glutamic acid 110 to Alanine)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. csd6_13-330_Y133A/pET28b(+) N-tag

   Plasmid

   #71356

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Tyrosine 133 to Alanine)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

6. csd6_13-330_C176A/pET28b(+) N-tag

   Plasmid

   #71360

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Cysteine 176 to Alanine)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

7. csd6_13-330_W158A/pET28b(+) N-tag

   Plasmid

   #71358

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Tryptophan 158 to Alanine)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. csd6_13-330_Y132A/pET28b(+) N-tag

   Plasmid

   #71355

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Tyrosine 132 to Alanine)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBullet-cyt-n

   Plasmid

   #53065

   Purpose

   destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

   Depositor

   Joshua Heazlewood

   Article

   Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ECFP and EYFP

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBullet-er-n

    Plasmid

    #53067

    Purpose

    destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBullet-tgn-n

    Plasmid

    #53071

    Purpose

    destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    Villin-mTQ2-P2A-FlpO-ERT2
    

    Use

    Tags

    mTQ2, linked to FlpO through an P2A ribosomal ski…

    Expression

    Mammalian

    Mutation

    Promoter

    Villin

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208048

    Purpose

    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    BirAopt
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BirAopt is human-codon-optimized BirA (PMID 15707…

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF6-Lck-TurboID

    Plasmid

    #159433

    Purpose

    expresses Lck-TurboID in mammalian cells

    Depositor

    Arthur Salomon

    Article

    Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

    Inserts

    Lck (LCK Human)
    TurboID (BirA mutant)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    EF-1a

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid

    #183502

    Purpose

    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    mKate2
    BSD
    

    Use

    Lentiviral and Luciferase

    Tags

    P2A linker and SV40 NLS with GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV-Sox10MCS5-GFP-EF1a-mCherry

    Plasmid

    #115782

    Purpose

    Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Inserts

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    EF1alpha promoter driven constitutive mCherry
    

    Use

    Lentiviral

    Tags

    EF1α promoter fused to mCherry and cfos promoter …

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMThy1.1_hDDX5_FL

    Plasmid

    #88873

    Purpose

    constitutive expression of DDX5 in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

    Plasmid

    #228254

    Purpose

    pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCAR_sgRNA_blast-tagBFP-lox5171

    Plasmid

    #162072

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge