Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,952 results for: Lif

Showing: 3101 - 3120 of 7952 results

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Plxnd1-AP-His

Plasmid

#72006

Purpose

Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnd1 (Plxnd1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb2(L)-AP-His

Plasmid

#72002

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS_PA-mEGFP-Tau

Plasmid

#255810

Purpose

mammalian expression of Tau tagged with PA and mEGFP

Depositor

Kristen Verhey

Article

Hotta et al Elife. 2026 Feb 9;14:RP109308. doi: 10.7

Insert

Tau (MAPT Human)

Tags

PA-tag, mEGFP

Expression

Mammalian

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS_internal PA-TubA1A-IRES-EGFP

Plasmid

#255804

Purpose

mammalian expression of PA-tagged human TubA1A(E254A). The PA tag (GVAMPGAEDDVV) is inserted between Ile42 and Gly43 in the flexible loop of TubA1A. An IRES drives expression of EGFP reporter.

Depositor

Kristen Verhey

Article

Hotta et al Elife. 2026 Feb 9;14:RP109308. doi: 10.7

Insert

TubA1A (TUBA1A Human)

Expression

Mammalian

Mutation

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a

Plasmid

#253172

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD2 ΔE6 3* Δr7a/VSVG

Plasmid

#253171

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a (residual AA);stop codon at AA213 in orf3a; VSV glycoprotein replaces orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with VSV-G

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/N1 PR8 Δ67a

Plasmid

#253169

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza N1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza N1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/H1 PR8 Δ67a

Plasmid

#253168

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza H1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza H1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔrE/N1 PR8 Δ67 3*

Plasmid

#253150

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope (residual AA), orf6, orf7a;stop codon at AA213 in orf3a; Influenza N1 (PR8) replaces env

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 PR8

Plasmid

#253147

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf6

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 WSN

Plasmid

#253146

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (WSN) replaces orf6

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 WSN

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE6 3* Δr7a/H1 PR8

Plasmid

#253151

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a (residual AA); stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 PR8

Expression

Bacterial

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔrE/N1 Δ6 3* Δ7a/H1 PR8

Plasmid

#253152

Purpose

Virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of env (residual AA), orf6, orf7a;stop codon at AA213 in orf3a; Influenza N1 (PR8) replaces envelope; H1 (PR8) replaces orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1/N1 PR8

Expression

Bacterial

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_CoV-2_frD2 ΔE6 3* Δr7a/H1 WSN

Plasmid

#253153

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of env, orf6, orf7a (residual AA);stop codon at AA213 in orf3a; Influenza H1 (WSN) replaces orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 WSN

Expression

Bacterial

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_CoV-2_frD2_ΔrE/N1 WSN Δ67 3*

Plasmid

#253154

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of env (residual AA), orf6, orf7a; stop codon at AA213 in orf3a; Influenza N1 (WSN) replaces envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 WSN

Expression

Bacterial

Available Since

May 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_CoV-2_ frD2_ΔE67 3* NA-P2A-N PR8

Plasmid

#253162

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza N1 (WSN)-P2A-N

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 PR8

Expression

Bacterial

Available Since

May 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_CoV-2_ frD2 ΔE6 3* Δr7a/N1 PR8

Plasmid

#253166

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a (residual AA);stop codon at AA213 in orf3a; Influenza N1 (PR8) replaces orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 PR8

Expression

Bacterial

Available Since

May 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_CoV-2_frD_Δ3E_GP LCMV Δ67a

Plasmid

#253179

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; LCMV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with LCMV GP

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge

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