We narrowed to 5,443 results for: SPI

1. pMK231 (AAVS1 CMV-MCS-PURO)

   Plasmid

   #105924

   Purpose

   Donor for integration at the human AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Thy1.1-GFP

   Plasmid

   #131703

   Purpose

   encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHEN2-SG4 R105A

   Plasmid

   #196072

   Purpose

   Express mutant (R105A) SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4 R105A
   

   Use

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Mutation

   R105A mutation in SG4

   Promoter

   T7

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-TPX2

   Plasmid

   #31227

   Depositor

   Patricia Wadsworth

   Article

   Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

   Insert

   TPX2 (TPX2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-coilin

   Plasmid

   #36906

   Depositor

   Greg Matera

   Article

   Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

   Insert

   coilin (COIL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLBH559_Tet-HisCas14a1Locus

   Plasmid

   #112502

   Purpose

   Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-IRES-GFP-mouse-Caspase-1

   Plasmid

   #183361

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEGFP-Cdc42-C1

   Plasmid

   #29673

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Nature. 2011 Mar 20. ():.

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1

    Plasmid

    #29676

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Mar 20. ():.

    Insert

    Pak3 (PAK3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa60-113 only; S74A F84A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDsRed1-N1 RanGAP

    Plasmid

    #13386

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-MP2

    Plasmid

    #36097

    Purpose

    3rd generation lentiviral plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESneo-PA-GFP-alpha Tubulin

    Plasmid

    #12297

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

    Plasmid

    #205277

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    C310R/D487K/T513K

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. betaRBPIG

    Plasmid

    #16422

    Depositor

    Juergen Hescheler

    Article

    Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

    Insert

    loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR-v2 sgATG7

    Plasmid

    #211534

    Purpose

    Deletes ATG7

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgATG7
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

    Plasmid

    #205268

    Purpose

    Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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