We narrowed to 10,458 results for: T7

1. pJKW0770

   Plasmid

   #119023

   Purpose

   E.coli expression plasmid for PmSPS1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmSPS1
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6CDG (GID4)

   Plasmid

   #119961

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 124-289

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. vgCam-pRSETb

   Plasmid

   #193362

   Purpose

   A FRET-based calcium probe using Sumire

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   vgCam
   

   Use

   E.coli expression

   Tags

   His X6 tag and Strep tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJKW0957

   Plasmid

   #119026

   Purpose

   E.coli expression plasmid for PmKOMT1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmKOMT1
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p_SNIPR_sensor_LacZ

   Plasmid

   #139464

   Purpose

   T7 RNAP-driven expression of SNIPR RNA with a LacZ reporter

   Depositor

   Alexander Green

   Article

   Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

   Insert

   SNIPR_lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTST6

   Plasmid

   #86603

   Purpose

   Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.

   Depositor

   Mary Ann Moran

   Article

   Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1

   Insert

   MTST6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. TAL2038

   Plasmid

   #39396

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-20-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pRSETb-tVYN-TmΔ

   Plasmid

   #149694

   Purpose

   High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

   Depositor

   Ming Hammond

   Article

   Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

   Insert

   VenusΔC12-TmYcgRΔ-NLucΔ4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2039

   Plasmid

   #39397

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-20-Right
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJKW0776

    Plasmid

    #119024

    Purpose

    E.coli expression plasmid for PmSPS2

    Depositor

    Jing-Ke Weng

    Article

    Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

    Insert

    PmSPS2
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJExpress414_GmPOPB

    Plasmid

    #92234

    Purpose

    Express wild type prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EcYbbN pET15b

    Plasmid

    #60690

    Purpose

    Recombinant protein expression of YbbN

    Depositor

    Mark Wilson

    Article

    Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

    Insert

    YbbN
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. A0A075HNX4

    Plasmid

    #177695

    Purpose

    expresses a fatty alcohol oxidase in E. coli

    Depositor

    Martin Engqvist

    Article

    Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

    Insert

    Long chain alcohol oxidase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-Streptavidin-Dead Aspartate Loop

    Plasmid

    #46368

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Dead Aspartate Loop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEPQDKN0248

    Plasmid

    #162313

    Purpose

    Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-sfGFP-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET3C-ARL16(CO)-His6

    Plasmid

    #216485

    Purpose

    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

    Depositor

    Richard Kahn

    Insert

    ADP-ribosylation factor-like 16 (ARL16 Human)
    

    Use

    Tags

    includes GSGSGSHHHHHH (His6) tag at C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HisFlag-CAD

    Plasmid

    #31615

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

    Insert

    CAD
    

    Use

    Tags

    3xFlag, His, and T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pUG-124

    Plasmid

    #104635

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
    

    Use

    Tags

    HIS-Ubq, HA-E2, GST-E3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MetNI (Lm)

    Plasmid

    #78316

    Purpose

    Methionine ABC Transporter

    Depositor

    Douglas Rees

    Article

    Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

    Inserts

    MetI
    MetN
    

    Use

    Tags

    10x Histidine Tag and 6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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